Mouse anti-Human CD34 Class-II (Endothelcells) (unconjugated), Clone QBEND/10, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E077
Category
Quantity
5 ml
Buy at BIOZOL

Background

In immunohistochemistry, the monoclonal antibody E071 stains mesothelial cells. It recognizes an antigen localized on the plasma membrane of mesothelial cells.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® CD34, Endothelial cells (Bottle, 5 ml)

Antigen

Purified human endothelial vesicles

Specificity

Human CD34 antigen (epitope neuraminidase-stable, glycoprotease- and chymopapain sensitive)

Clone

QBEND/10

Isotype

Mouse lgGM

The antibody clone QBEND/1 reacts with an epitope on endothelial cells and hematopoietic progenitor cells that is not neuraminidase sensitive but is cleaved by chymopapain and glycoproteases (class II antibody). It can be used to detect endothelial cells and hematopoietic progenitor cells in tissue, cell smear and cell cultures.

Pretreatment

Pre-treatment with HISTOSAFE-ENHANCER (Art. No. E7000) for paraffin sections. No protease treatment.

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Tonsille

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Civin C.I., Strauss L.C., Brovall C., Fackler M.J. , Schwartz J.F., Shaper J.H. (1984) Antigenic analysis of haematopoesis. III. A hematopoetic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-Ia cells. J. Immunol. 133; 157-165.
  • Civin C.I., Trischmann T.M., Fackler M.J., Bernstein I.D., Bühring H.J., Campos L., et al. (1989) M7.1. Report on the CD34 cluster workshop. In: Kapp W. et al. Eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Press pp. 818-8
  • Fina L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75; 2417-2426.
  • Dercksen M.W., Daams G.M., de Haas M., von dem Borne A.E.G.K., and van der Schoot C.E. M10.2. Charakterization of the CD34cluster. In: Schlossman S.F. et al. Eds. Leucocyte V. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Typing Press pp. 850-853.
Specificity

CD34 Class-II (Endothelcells)
Species Reactivity

Human, Monkey, Rhesus Monkey
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

QBEND/10
Antigen

Human CD34 Class-II (Endothelcells)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet