Mouse anti-Human Cytokeratin-14 (CK14) (unconjugated), Clone LL002, IgG3, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K078
Category
Quantity
0, 5 ml

Background

Cytokeratin 14 is found in stratified epithelial cell types but not simple epithelial. This antibody with help detect cytokeratin 14 and identify uncharacertized tissues.

Volume/Quantity

0.5 ml

Product Form

Tissue culture supernatant – liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)

Immunogen

Synthetic peptide of 15 amino acids representing the end C-terminal region of human keratin 14.

Clone Number

LL0002

Isotype

IgG3 (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/20
Immunohistology-paraffin Yes 1/20
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product requires heat-induced epitope retrival.

Positive Control

Skin. Cytoplasmic staining pattern.

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG3 Negative Control (LINARIS CatNo ITC2063)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Lane E B and Alexander C M. Use of keratin antibodies in tumour diagnosis. Cancer Biology. 1: 165- 179 (1990).

Purkis P E, Steel J B, Mackenzie I C, et al.. Antibody markers of basal cells in complex epithelia. Journal of Cell Science. 97: 39-50 (1990).

Westzels R H W, Holland R, Van Haelst U J G M, et al.. Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast. American Journal of Pathology. 134(3): 571-579 (1989).

Moll R, Franke W W, Schiller F L, et al.. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumours and cultured cells. Cell. 31: 11-24 (1982).

Specificity

Cytokeratin-14 (CK14)

Species Reactivity

Human, Mouse, Rat

Host / Source

Mouse

Isotype

IgG3

Application

IHC-F, IHC-P

Clone

LL002

Antigen

Human Cytokeratin-14 (CK14)

Quantity

0, 5 ml

Format

Purified

Storage Temperature

2-8 °C

Shipping Temperature

2-8 °C

Documents

pdf Datasheet