Mouse anti-Human Cytokeratin-7 (CK-7) (unconjugated), Clone OV-TL 12/30, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E061
Category
Quantity
5 ml
Buy at BIOZOL

Background

Part of the cytoskeleton of epithelia and cells derived from them consists of cytokeratins. These interrelated polypeptides are divided into two groups, acidic and basic peptides. To date, 20 cytokeratins have been characterized in more detail and their expression in different cell types has been studied. The molecular weights range from 40 to 69 kDa. Each epithelial tissue shows a specific and stable expression pattern of cytokeratins.

A nomenclature of cytokeratins used for immunohistochemical studies was outlined by Moll et al. (1982). Cytokeratin 20 was discovered and characterized at a later time (Moll et al. 1990 and 1992). In the differential diagnosis of carcinomas of the abdomen, cytokeratin 7 and cytokeratin 20 are highly complementary.

Normal Tissues

The monoclonal antibody E061 reacts with cytokeratin 7 in numerous epithelia of glands and glandular ducts including bile ducts and transistorized epithelia of the urinary tract. Most notably, epithelia of the lung and breast are visualized with this antibody. The antibody does not react with prostate epithelia, colonic epithelia, squamous epithelia, hepatocytes and other nonepithelial cells. Of course, it also does not react with other cytoskeletal proteins.

Abnormal Tissues

Neoplasms that express CK-7 are derived from tissues that also normally produce CK-7. These include specific adenocarcinomas of the ovary, breast and lung, and transitional cell carcinoma.
Gastrointestinal carcinomas and prostate carcinomas are usually negative, as are squamous cell carcionomas, making the antibody a relatively good marker for adenocarcinoma and transitional cell carcinoma (Ramaekers et al., 1990).
Small cell lung carcinoma (SCLC) and endocrine tumors of the intestinal tract and pancreas are also negative. With adenocarcinomea of the lung, breast, and non -mucinous carcinomas of the ovary negative.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin 7 (Bottle, 5 ml)

Antigen

Ovarian carcinoma cell line OTN 11 (Poels et al., 1989).

Specificity

Human cytokeratin 7, cross-reaction with animal cytokeratins not studied.

Abnormal Tissue

Differential diagnosis adenocarcinomas (lung, breast, ovary) and their metastases *.

Clone

OV-TL 12/30

Isotype

Mouse lgG1

Normal Tissues

The monoclonal antibody E061 reacts with cytokeratin 7 in numerous epithelia of glands and glandular ducts including bile ducts and transistorized epithelia of the urinary tract. Most notably, epithelia of the lung and breast are visualized with this antibody. The antibody does not react with prostate epithelia, colonic epithelia, squamous epithelia, hepatocytes and other nonepithelial cells. Of course, it also does not react with other cytoskeletal proteins.

Abnormal Tissues

Neoplasms that express CK-7 are derived from tissues that also normally produce CK-7. These include specific adenocarcinomas of the ovary, breast and lung, and transitional cell carcinoma. Gastrointestinal carcinomas and prostate carcinomas are usually negative, as are squamous cell carcionomas, making the antibody a relatively good marker for adenocarcinoma and transitional cell carcinoma (Ramaekers et al., 1990).
Small cell lung carcinoma (SCLC) and endocrine tumors of the intestinal tract and pancreas are also negative. With adenocarcinomea of the lung, breast, and non -mucinous carcinomas of the ovary negative.

Pretreatment

Pretreat deparaffinized sections with Pronase 0.1 % (LINARIS CatNo E110) for 10 minutes at RT or Histosafe-Enhancer (LINARIS CatNr E7000) for 10 minutes at 96-100°C.

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Breast Cancer

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Moll R., Franke W. W., Schiller D. L. Geiger B., and Krepler R. The catalog of human cytokeratin polypeptides: Pattern of expressions of specific cytokeratins in normal epithelia, tumors and cultured cells. Cell 31; 11-24 (1982)
  • Poels I. G., Jap P H. K., Ramaekers F. C. S., Scheres J. M. J. C., Thomas C. M. G., Vooijs P. G. Characterization of a hormoneproducing ovarian carcinoma cell line. Gynecologic Oncology 32; 203-2114 (1989)
  • Ramaekers F., van Niekerk C., Poels L., Schaafsma E., Huijsmans A., Robben H. Use of monoclonal antibodies to cytokeratin 7 in differential diagnosis of adenocarcinomas. Am. J. Pathol. 136; 641-655 (1990)
  • Moll R., Schiller D. L., Franke W. W. Identification of protein IT of the intestinal cytoskeleton as a Novel type I cytokeratin with unusual properties and expressions patterns. J. Cell Biol. 111; 567-580 (1990)
  • Moll R., Löwe A., Läufer J., and Franke W. W. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am. J. Pathol. 140; 427-447 (1992)
  • Van de Molenkraft F. J. J. M., van Niekerk C. C., Jap P. H. K., Poels L. G. OV-TL 12/30 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer. Histopathology 22: 35-38 (1993)
Staining of Human Lung with Mouse anti-Human Cytokeratin 7 Clone OV-TL 12/30
Specificity

Cytokeratin-7 (CK-7)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

OV-TL 12/30
Antigen

Human Cytokeratin-7 (CK-7)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet