Mouse anti-Human Epithelial Membrane Antigen (EMA) (unconjugated), Clone E29, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E049
Category
Quantity
5 ml
Buy at BIOZOL

Background

Epithelial membrane antigen belongs to a group of homomolecular glycoproteins isolated from the membrane of human milk fat globules. These proteins are also known as human milk fat globulins (HMFG). Their molecular weight ranges from 265 to 400 kDa. In addition to milk, these proteins are found in a number of normal epithelia as well as in tumors derived from them.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Epithelial Membrane Antigen (Bottle, 5 ml)

Antigen

Epithelial membrane antigen

Specificity

Human Epithelial Membrane Antigen (EMA)

Clone

E29

Isotype

Mouse lgG2a

Fusion Partners

The monoclonal antibody was obtained by immunizing mice with human milk fat globulin and fusing the spleen cells with mouse myeloma cells.

The E049 antibody reacts with a variety of human epithelial cells. In epithelial tumors, this marker is also strongly expressed. Thereby, mammary carcinomas show the strongest expression. Moreover, the antibody is particularly reactive in tumors of glandular origin (e.g. sweat glands). The antigen can also be detected in squamous cell carcinomas, even if the epithelium of origin (e.g. epidermis) itself is not reactive. In contrast, non-epithelial tumors do not contain detectable EMA.
The antibody also detects some lymphoid neoplasms, especially CD30-positive large cell lymphomas.

Pretreatment

Proteolytic pretreatment of the kerosene sections is not required.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Breast Cancer

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Heydermann, E., Strudly I., Powell G., Richardson T. C., Cordell J. L., and Mason D. Y. (1985). A new monoclonal antibody to epithelial membrane antigen (EMA) – E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody HMFG-2. Br. J. Cancer 52; 355-361.
  • Delsol G., Al Saati T., Gatter K. C., Gerdes J., Schwarting R., Caveriviere P., et al. (1988). Coexpression of epithelial membrane antigen (EMA), Ki-1, and Interleukin-2 receptor by anaplastic large cell lymphomas. Am. J. Pathol. 130; 59-70.
  • Wick M. R., Swansson P. E., and Manivel J. C. (1988). Immunhistochemical findings in tumors of skin. In: De Lellis R. A. ed. Advances in Immunohistochemistry, Raven Press, New York pp. 395-429.
  • Russo J. and Russo I. H. (1988). Immuncytochemical markers in breast cancer. In. De Lellis R. A. ed Advances in Immunhistochemistry, Raven Press, New York pp. 431-475.
Specificity

EMA
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

E29
Antigen

Human EMA
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet