Mouse anti-Human Estrogen Receptor (unconjugated), Clone 1D5, IgG, Ready-to-use

The monoclonal antibody E051 reacts with the N-terminal sequence of the human estrogen receptor (ER). It shows nuclear staining of epithelia of the uterus and mammary glands.
Specifically, estrogen receptors are experimented in the nuclei of the corresponding carcinomas. Tissue-dependent cytoplasmic staining may also occur.
The antibody can be used for frozen sections as well as for formalin-fixed kerosene material. A prerequisite for satisfactory staining results is a first-class preservation status of the tissue. However, recent investigations by our development laboratory have shown that differences in fixation can be largely compensated for by pretreating the tissue sections with our HistoSafeENHANCER (LINARIS CatNo E7000). After this pretreatment, a one-hour incubation period with the primary antibody (just as with frozen sections) is sufficient.
In addition, we offer other paraffin-embedded antibodies such as anti-progesterone receptor (LINARIS CatNo E054), anti-cathepsin D (LINARIS CatNo E048) or anti-p-53 (LINARIS CatNo E052) for questions on breast carcinoma, fo

Pretreatment

Pretreat paraffin sections for 20 min at 96-100°C with HISTOSAFE ENHANCER (LINARIS CatNo E7000) in a water bath or 3 times 6 min in the microwave. Cryostat material does not require pretreatment!

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Breast Cancer

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).