Mouse anti-Human Vimentin (unconjugated), Clone VIM3B4, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E024
Category
Quantity
6 ml
Buy at BIOZOL

Background

Vimentin is an intermediate filament with a relative molecular mass of 57kD. It forms, together with microfilaments and microtubules, the cytoskeleton of non-epithelial cells, e.g. non-muscle mesenchymal cells and cells of mesenchymal origin including vascular smooth muscle, astrocytes and numerous cultured cell lines. It frequently co-occurs with other intermediate filaments such as desmin.

Expression of this protein is retained during the conversion of normal to neoplastic cells.

Synonyms

Vimentin

Reagents sufficient for about 60-120 tissue sections

  • 1 dropper bottle HISTOPRIME® Vimentin (Bottle, 6 ml )

Antigen

Purified vimentin from bovine lentils

Specificity

Human vimentin

Abnormal Tissue

Tumor of mesenchymal origin such as sarcomas, myosarcomas, lymphomas, melanomas.

Clone

VIM 3B4

Isotype

Mouse lgG2a

Fusion Partners

BALB/c mice were immunized with vimentin (from bovine lentils). Spleen cells from these animals were fused to a tumor cell line. Ascites-derived antibodies were purified by affinity chromatography on protein A-Sepharose.

The monoclonal antibody E024 (VIM 3B4) reacts with human vimentin as well as with vimentin in mouse, rat, bovine, chicken and amphibian. It recognizes tumors of mesenchymal origin, e.g., sarcomas including myosarcomas, lymphomas, and melanomas. Proteolytic pretreatment is required after formaldehyde fixation. Alternative without pronase treatment: LINARIS CatNo E034.

Pretreatment

For formaldehyde-fixed kerosene material, apply 0.1% Pronase (LINARIS CatNo E110) for 10min at room temperature.

Incubation Period

1 hour at room temperature after pretreatment with pronase. (Overnight incubation at 37°C necessary if no proteolytic pretreatment was performed!)

Usage

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

Control Tissue

Prostate or appendix

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Franke W.W., E. Schmid, S. Winter, M.Osborne, K.Weber, Exp. Cell Res. 25-46 (1979)
  • Lazarides E., Nature 283; 249-256 (1980)
  • Lazarides E., Ann. Rev. Biochem. 51; 219-250 (1982)
  • Osborn M., K.Weber, Laboratory Investigation 48, 372-394 (1983)
  • Toelle H.-G., K. Weber, M. Osborn, M. Eur. Cell Biol. 38; 234-244 (1985)
Specificity

Vimentin
Species Reactivity

Amphibien, Bovine, Chicken, Hamster, Human, Mouse, Rat
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

VIM3B4
Antigen

Human Vimentin
Quantity

6 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet