Rabbit anti-Human CD3 (T-Lymphocytes), Polyclonal, IgG, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E053
Category
Quantity
5 ml
Buy at BIOZOL

Background

The CD3 complex is experimented on the cell membrane of T lymphocytes. The complex consists of several protein subunits (including gamma, delta, epsilon) associated with the T cell receptor (TCR). The CD3- molecules are involved in signal transduction after antigen binding to the TCR.

CD3 is experimented by cortical thymocytes and by peripheral T lymphocytes. No other peripheral cells experiment CD3 (except possibly Purkinje cells in the cerebellum).

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® CD3, T cells (Bottle, 5 ml)

Antigen

Prokaryotic fusion protein corresponding to the human CD3 epsilon chain.

Specificity

Epsilon subunit of the human CD3 complex Collagen IV alpha! AND/or alpha 2-chain in
connective tissue, basement membranes

Abnormal Tissue

T-cell lymphomas

Clone

Polyclonal

Isotype

Rabbit Ig

Fusion Partners

Synthetic human epsilon-chain CD3 peptide (AS 156-168) was used to immunize rabbits after coupling to thyroglobulin. Anti-CD3 antibodies were isolated from rabbit serum by affinity chromatography on immobilized CD3

The polyclonal antibody E053 recognizes the cytoplasmic part of the epsilon subunits of the CD3 complex on thymocytes and on peripheral T lymphocytes and, in contrast to many monoclonal antibodies of the same specificity, is suitable for cryostat material and kerosene sections.

The E053 antibody is predominantly used for the differential diagnosis of T-cell lymphomas:

Pretreatment

Enzymatic pretreatment with 0.01% Pronase (LINARIS CatNo E110) 10 min at room temperature or better HISTOSAFE-ENHANCER (LINARIS CatNo E7000) 20 min at 96°C

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Tonsils

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Rabbit IgG (Vector CatNo AK-5001) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Rabbit IgG (Vector CatNo PK-6101) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Chetty R and Gatter K. CD3: structure, function, and role of immunostaining in clinical practice. Journal of Pathology. 173: 303-307 (1994).
  • Clark E A and Ledbetter J A. Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. Immunology Today. 10: 225-28 (1989).
  • Clevers H, Alarcon B, Wileman T, et al.. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Review of Immunology. 6: 629-662(1988).
  • Clevers H, Dunlap S and Terhorst C. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. European Journal of Immunology. 18: 705-710 (1988).
  • Campana D, Thompson J S, Amlot P, et al.. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. Journal of Immunology. 138: 648-655 (1987).
  • Denning S M, Tuck D T, Singer K H, et al.. Activation of human thymocytes via CD3 and CD2 molecules. In: McMichael AJ, et al.. eds. Leucocyte Typing III, White Cell Differentiation Antigens. Oxford-New York-Tokyo. Oxford University Press. 144-147 (1987).
  • Meuer S C, Acuto O, Hussey R E, et al.. Evidence for the T3-associated 90k heterodimer as the T cell antigen receptor. Nature. 303: 808-810 (1983).
  • Beverley P C L and Callard R E. Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. European Journal of Immunology. 11: 329-334 (1981).
Specificity

CD3 (T-Lymphocytes)
Species Reactivity

Human
Host / Source

Rabbit
Isotype

IgG
Application

IHC-F, IHC-P
Clone

Polyclonal
Antigen

Human CD3 (T-Lymphocytes)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet