Mouse anti-Human CD10 (CALLA) (unconjugated), Clone 56C6, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E084
Category
Quantity
6 ml

Background

The CD10 antigen (CALLA, Common Acute Lymphoblastic Leukemia Antigen) is an integral type II membrane protein with a molecular weight of 100 kDa. CD10 has been identified as a human membrane associated neutral metallo-endopeptidase. Other names include NEP, encephalokinase, or neprilysin. CD10 is expressed by immature B lymphocytes, some immature T lymphocytes, mature granolucocytes, approximately 75% of B-ALL, some T-ALL/TLBL, and all ALL subtypes, among others. Burkitt’s lymphoma and myeloma are also positive for CD10, as are some diffuse large B-cell lymphomas and most follicular lymphomas. Also positive are some tumors of epithelial origin such as carcinomas of the kidney, urinary bladder, prostate, uterus, and liver. Those negative for CD10 include MALT lymphomas and mantle cell lymphomas. CD10 is a commonly used marker for differential diagnosis of lymphoma, but can also be used for further differentiation, e.g., hepatocellular carcinoma vs. liver metastases of other origin.

Reagents sufficient for about 60-120 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin 14 (Bottle, 6 ml)

Antigen

Recombinant protein corresponding to the external domain of human CD10.

Abnormal Tissue

CD10 is a commonly used marker for differential diagnosis of lymphoma, but can also be
used for further differentiation, e.g. hepatocellular carcinoma vs. liver metastases of other
origin.

Clone

56C6

Isotype

Mouse lgG1

The antibody shows a positive staining result in the cell membrane of CD10-positive cells in formalin-fixed, paraffin-embedded tissue.

Pretreatment

Microwave treatment Citrate buffer pH 6.0

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

Specificity

CD10 (CALLA)

Species Reactivity

Human

Host / Source

Mouse

Isotype

IgG1

Application

IHC-F, IHC-P

Clone

56C6

Antigen

Human CD10 (CALLA)

Quantity

6 ml

Format

RTU

Storage Temperature

2-8 °C

Shipping Temperature

20 °C

Documents

pdf Datasheet