Mouse anti-Human CD45RO (unconjugated), Clone UCH-L1, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
K027
Category
Quantity
0, 1 mg
Buy at BIOZOL

Background Human CD45RO

The monoclonal antibody E027 (UCHL1) recognizes a T cell-associated antigen with a molecular weight of approximately 180 kDA. This is the low molecular weight isoform of the leucocyte common antigen (LCA) CD45, which since the IV International Workshop on Leucocyte Differentiation Antigens (Vienna 1989) has been designated CD45RO). The antigen is expressed by a functional subset of CD4+ T cells, by most thymocytes, and by granulocytes and monocytes. Studies of B- and T-cell lymphomas and plasmocytes suggest that CD45RO (UCHL1) is expressed in normal B cells prior to differentiation to plasma cells, but plasmocytomas are negative except for a few reactive lymhpozytes. Negative for CD45RO are also cutaneous T-cell lymphomas (mostly CD4+, CD45R-).

Synonyms

T cell Marker, CD45RO, UCHL1

Volume/Quantity

0.1 mg

Product Form

Purified IgG – liquid

Clone Number

UCHL1

Buffer

Phosphate buffered saline

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Preservatives Stabilisers

0.09 Sodium Azide (NaN3)

Immunogen

Human IL-2 dependent T cells

Isotype

IgG2a (Mouse)

Specificity

Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.
In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

Suggested Working Dilution

FlowCytometry Yes 1/25-1/50
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/100-1/300
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested

Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Tonsil

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

CD45RO
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

UCH-L1
Antigen

Human CD45RO
Quantity

0, 1 mg
Storage Temperature

2-8 °C / -20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet