Mouse anti-Human Chromogranin A (unconjugated), Clone LK2H10, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K001
Kategorie
Quantity
0, 1 mg

Background

Hormone-producing organs and various hormone-producing cells in non-endocrine cells form the endocrine system. These hormone-producing cells are also referred to as the „diffuse neuro-endocrine system“ (DNS)1, in which biogenic amines are both stored and decarboxylated (APUD concept)1. Although there are major functional differences among DNA cells due to the production of different hormones, they have in common the presence of chromogranin in the secretory granules.

Initially, chromogranin was identified as the major secretory protein of medullary chromaffin cells of the adrenal gland². However, in more recent immunohistochemical studies, it has been recognized as a widespread marker of almost all granule-containing endocrine cells and the tumors arising from them³-6. The behavior of tumor staining is proportional to the number of secretory storage granules present. Most endocrine tumors exhibit strong staining with the exception of small cell lung carcinoma, Merkel cell carcinoma, and neublastomas, which (with E001) assume only weak staining.

Volume/Quantity

100 µg/100 µl

Product Form

Purified IgG – liquid

Clone Number

LK2H10

Preparation

Ammonium sulphate precipitation followed by ion exchange chromatography.

Buffer

Phosphate buffered saline

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Preservatives Stabilisers

10 mM Sodium Azide (NaN3)

Immunogen

Human pheochromocytoma tissue

Isotype

IgG1 (Mouse)

Specificity

K001 recognises human Chromogranin A and related chromogranin polypeptides. The antibody stains
endocrine derived cells containing secretory storage granules. In staining of tumour cells the intensity of
staining is proportional to the number of secretory storage granules present. Small cell carcinoma of the
lung, Merkel cell carcinomas and neuroblastomas exhibit weak staining, whilst most other endocrine
derived tumours stain strongly.

Fusion Partners

Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse NSI myeloma cell
line.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/1000
Immunohistology-paraffin Yes 1/1000
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product does not require protein digestion pre-treatment of paraffin sections

Positive Control Tissue

Normal Pancreas

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

DeStephano, D.B., Lloyd, R.V., Pike, A.N., Wilson, B.S. (1984). Pituitary Adenomas: An immunohistochemical study of hormone production and chromogranin. Am. J. Pathol. 116: 464.

Lloyd, R.V. and Wilson, B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody. Science 222: 628

Lloyd R.V., Mervak T., Schmidt K., Warner T., Wilson B.S. (1984). Immunohistochemical detection of chromogranin and neuron-specific enolase in pancreatic endocrine neoplasms. Am. J. Surg. Pathol.
8: 607.

Walts, A.E., Said, J.W., Shintaku, P. and Lloyd, R.V.: (1985). Chromogranin as a marker of neuroendocrine cells in cytologic material – an immunocytochemical study. Am. J. Clin. Pathol. 84: 273-277.

Wilson, B.S., and Lloyd, R.V. (1984). Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115: 458.

Specificity

Chromogranin A

Species Reactivity

Human, Monkey, Simian, Swine

Host / Source

Mouse

Isotype

IgG1

Application

IHC-F, IHC-P

Clone

LK2H10

Antigen

Human Chromogranin A

Quantity

0, 1 mg

Format

Purified

Storage Temperature

2-8 °C / -20 °C

Shipping Temperature

2-8 °C

Dokumente

pdf Datasheet