Mouse anti-Human Cytokeratin-14 (CK14) (unconjugated), Clone LL002, IgG3, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E078
Category
Quantity
5 ml
Buy at BIOZOL

Background

The antibody clone QBEND/1 reacts with an epitope on endothelial cells and hematopoietic progenitor cells that is not neuraminidase sensitive but is cleaved by chymopapain and glycoproteases (class II antibody). It can be used to detect endothelial cells and hematopoietic progenitor cells in tissue, cell smear and cell cultures.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin 14 (Bottle, 5 ml)

Antigen

C-terminal 15-AS peptide of cytokeratin 14 conjugated to thyroglobulin.

Specificity

Human Cytokeratin 14

Clone

LL002

Isotype

Mouse lgG3

The cytokeratin 14 antibody is suitable for distinguishing squamous epithelia from simple epithelia that do not express this CK.

Pretreatment

Pre-treatment with HISTOSAFE-ENHANCER (LINARIS CatNo E7000) for paraffin sections.

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Skin

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).

Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.
  • Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.
Specificity

Cytokeratin-14 (CK14)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG3
Application

IHC-F, IHC-P
Clone

LL002
Antigen

Human Cytokeratin-14 (CK14)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet