Mouse anti-Human Cytokeratin-18 (CK18) (unconjugated), Clone Cy90, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K041
Category
Quantity
0, 1 ml
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Background

Epithelial cells and their derivatives typically consist of intermediate filaments composed of approximately 20 different related polypeptides. The molecular weights of these cytokeratins range from 40,000 to 69,000 Da. Each epithelial tissue shows a specific and stable expression pattern of these cytokeratin subunits. Tumors originating from epithelia retain the cytokeratin expressions of normal epithelial cells. For this reason, carcinomas can be identified and classified using antibodies that specifically react with the various cytokeratins.

Volume/Quantity

0.1 ml

Product Form

Ascites – liquid

Preservatives Stabilisers

0.09 Sodium Azide (NaN3)

Immunogen

Human A-431 and MCF-7 cell lines

Clone Number

Cy90

Isotype

IgG1 (Mouse)

Specificity

The monoclonal antibody K041 (CY-90) reacts highly specifically with human cytokeratin18 in a variety of simple epithelia (e.g. with intestinal, bronchial, urinary tract, liver and glandular epithelia of prostate and uterus).
It does not react with squamous epithelia of the following organs: epidermis, hair follicles, sebaceous glands, tongue, esophagus and vagina or with non-epithelial cells.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/50-1/100
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Positive Control Tissue

Tonsil

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Cytokeratin-18 (CK18)
Species Reactivity

Bovine, Human, Rat
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

Cy90
Antigen

Human Cytokeratin-18 (CK18)
Quantity

0, 1 ml
Format

Purified
Storage Temperature

-20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet