Mouse anti-Human Cytokeratin-20 (CK20) (unconjugated), Clone Ks 20.8, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

5 ml


Part of the cytoskeleton of epithelia and cells derived from them consists of cytokeratins. These interrelated polypeptides are divided into two groups, namely acidic and basic peptides. To date, 20 cytokeratins have been characterized in more detail and their expression in different cell types has been studied. The molecular weights range from 40 to 69 kDa. Each epithelial tissue shows a specific and stable expression pattern of cytokeratins.

A nomenclature of cytokeratins used for immunohistochemical studies was outlined by Moll et al. (1982). Cytokeratin 20 was discovered and characterized at a later date (Moll et al., 1990 and 1992).

Normal Tissues

The monoclonal antibody E062 reacts with cytokeratin 20 in epithelia of the intestine, gastric wall, paracrine cells of the pylorus, urether epithelium, and Merkel cells of the epidermis. Most other epithelia, including epithelia of the mammary glands, are CK-20 negative.

Abnormal Tissues

The expression of CK-20 varies with the type of carcinoma. Neoplasms that express CK-20 are always derived from tissues that also normally produce CK20. Colon rectal carcinomas regularly produce CK-20, while this is true to a lesser extent for adenocarionms of the stomach.
It is also expressed by adenocarcinomas of the gallbladder, bile ducts, pancreas, and mucinous ovarian carcinomas. Particularly intense staining is obtained in Merkel cell carcinomas of the skin.

Adenocarcinomas of the breast, lung, endometrium, and nonmucinous tumors of the ovary are usually negative. Also negative are small cell lung carcinomas (SCLC) and endocrine tumors of the intestinal tract and pancreas.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin 20 (Bottle, 5 ml)


Cytoskeletal proteins from microvilli of human duodenal mucosa.


Human cytokeratin 20 (according to Moll et al.)

Abnormal Tissue

Differential diagnosis of intestinal carcinomas and their metastases Merkel cell carcinomas.


Ks 20.8


Mouse lgG2a, kappa


Deparaffinized sections with Pronase 0.1% (LINARIS CatNo E110) for 10 minutes at RT or HistoSafe Enhancer (LINARIS CatNo E7000) for 10 minutes at 96-100 °C in a water bath.

Incubation Period

1 hour at room temperature


Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Appendix or colon carcinoma

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Moll R., Franke W. W., Schiller D. L., Geiger B., and Krepler R. The catalog of human cytokeratin polypeptides. Pattern of expressions of specific cytokeratins in normal epithelia, tumors and cultured cells. Cell 31; 11-24 (1982)
  • Moll R., Schiller D. L., Franke W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properities and expressions patterns. Cell Biol. 111; 567-580 (1990)
  • Moll R., Löwe A., Läufer J., and Franke W. W. Cytokeratin 20 in human carzinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am. J. Pathol. 140; 427-447 (1992)

Cytokeratin-20 (CK20)

Species Reactivity


Host / Source







Ks 20.8


Human Cytokeratin-20 (CK20)


5 ml



Storage Temperature

2-8 °C

Shipping Temperature

20 °C


pdf Datasheet