Mouse anti-Human Cytokeratin-20 (unconjugated), Clone Ks 20.8, IgG2a, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K062
Category
Quantity
0, 5 ml
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Background

K062 antibody reacts with the 46 kD cytokeratin intermediate filament protein as indicated by immunoblotting of cytokeratin-enriched cytosketal preparations isolated from villi of duodental mucosa.
Colrectal carcinomas consistenly expressed cytokeratin 20 while adenocarcinomas of the stomach expressed cytokeratin 20 to a less degree. Further adenocarcinomas of the gal bladder and bile ducts, ductal cell adenocarcinomas of the pancreas, mucinous ovarian tumours and transitional-cell carcinomas have been found to stain positively with the antibody. Most of the carcinomas from other sides were not positive using the antibody to cytokeratin 20, e.g. adenocarcinomas of the breast, lung and endometrium and non-mucinous tumours of the ovary. A consistently strong staining of Merkel cell carcinomas of the skin with the anti-cytokeratin 20 antibody was seen. This was in striking contrast to the lack of positivity in small cell lung carcinomas and in intestinal and pancreatic neuroendocrine tumour cells.

Volume/Quantity

0.5 ml

Product Form

Tissue culture supernatant – liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer

Phosphate buffered saline, pH7.4

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)
0.5% BSA

Immunogen

Cytoskeletal preparation isolated from microdissected villi of human duodental mucosa.

Clone Number

Ks20.8

Isotype

IgG2a (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/25-1/100
Immunohistology-paraffin Yes 1/25-1/100
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes 1/1000-1/5000
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product requires protein digestion pre-treatment of paraffin sections e. g. Pronase (LINARIS CatNo E110) or heat-induced epitope retrival.

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG2a Negative Control (LINARIS CatNo ITC0929)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Moll R, et al (1990). Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell biol 111: 567 – 580.

Moll R; et al (1992). cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 140: 427 – 447.

Moll R, et al. (1982). The catalog of human cytokeratins : patterns of expression in normal epithelia, tumors, and cultured cells. Cell 31: 11 – 24.

Specificity

Cytokeratin-20 (CK20)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

Ks 20.8
Antigen

Human Cytokeratin-20 (CK20)
Quantity

0, 5 ml
Format

Purified
Storage Temperature

2-8 °C / -20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet