Mouse anti-Human Cytokeratin Pan (unconjugated), Clone AE1+AE3, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

0, 1 ml


A group of 19 different water-insoluble proteins forms the cytokeratins. Together with microtubules and microfilaments, they form the cytoskeleton of epithelial cells. Normally, it is the case that different cytokeratins are found simultaneously in one cell. This is independent of cell type, cell environment, disease and differentiation stage.

If a conversion from normal to neoplastic cells takes place, the expression of these proteins is maintained.


Cytokeratin Pan


100 µg/100 µl

Clone Number


Product Form

Purified IgG – liquid


Borate buffered saline pH8.0


Monoclonal antibodys were collected from the ascites and purified by sodium sulphate precipitation.

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 1 mg/ml


Human epidermal keratins


IgG1 for both (Mouse)


Keratins are a group of water-insoluble proteins that form monofilaments, a´class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (1). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. Anti-Keratin AE1 recognizes the 56.5, 50, 50’, 48, and 40 kD keratins of the acidic subfamily. Anti-Keratin AE3 recognizes all members of the basic subfamily. Anti-Keratin AE1 And AE3 monoclonal antibodies have been used to characterize the source of various neoplasms and to study the distribution of keratin-containing cells in epithelia during normal development and during the development of epithelial neoplasms (8-13).

Fusion Partners

Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse P3 myeloma cell line

Suggested Working Dilution

FlowCytometry Not tested 0.5 – 1µg x 106 cells
Immunohistology-frozen Yes 0.5 – 2 µg/ml
Immunohistology-paraffin Yes 0.5 – 2 µg/ml
Immunofluorescence Yes 1 – 2 µg/ml
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.



Proteolytic (0.1% Pronase LINARIS CatNo E110) treatment enhances specific staining of formalin-fixed
paraffin-embedded tissue sections and allows higher dilutions of the antibody to be used (14).

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C.

Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R. (1982) Cell 31:11.

Sun, T.T., Eichner, R., Cooper, D. Schermer, A., Nelson, W.G. and Weiss, R. A. (1984) The Cancer Cell 1:169.

Cooper, D., Schermer, A. and Sun, T, T. (1985) Lab Invest. 52,243.

Sun, T. T. Tseng, S. C.G., Huang, A.J.W., Cooper, D. Lynch, M.H., Weiss, T., Eichner, R. and Schermer. (1985) “Monoclonal Antibody Studies of Keratin Expression: A Review” in Intermediate Filaments (Wang, E. et al. eds.) Vol 455, p. 307, New York Acadamy of Sciences.

Weiss, R.A., Eichner, R. and Sun, T.T. (1984) J. Cell Biol. 98:1397.

Woodcock-Mitchel, J. and Sun, T.T. (1982) Cell 30:361.

Tseng, D.C.G., Jarvinen, M.J., Nelson, W.G., Huang, J. W., Woodcock- Mitchel, J. and Sun, T.T. (1982) Cell 30:361.

Asch, B.B. and Asch, H.L. (1986) Cancer Research. 46:1255.

Rodriguez, M.M., Krachmer, J.H. and Sun, T.T. (1986) Trans Am. Opthalmo. Soc. 84:146.

Clausen, H. Vedtofte, P. Moe, D., Dabelsteen, E. Sun, T.T. and Dale, B. (1986) J. Invest. Dermatol. 86:249.

Laster, J.J., Itoh, T., Palker, T.J. and Haynes, B.F. (1986) Differentiation 31:67.

Klein-Szanto, A.J., Boysen, M. and Reith, A. (1987) Arch. Pathol. Lab. Med. 111:1057.

Reibel, J., Scholdt, M. and Dabelsteen, E. (1985) Acta Pathol.Microbiol. Immunol. Scand. 93; 159.

Pinkus, G.S., O’Conner, E.M., Etheridge, C.L. and Carson, J.M. (1985) J. of Histochemistry and Cytochemistry 333:465.

Hsu, S.M., Raine, L. and Fanger, h. (1981) Am. J. Clin. Pathol. 75:734.

Falini, B. and Taylor, C.R. (1983) Arch. Pathol. Lab. Med. 107:105.

Harlow, E. and Lane, D. (1988) Anhtibodies: A Laboratory Manual p. 359, Cold Spring Harbor, NY.

Taylor, C.R. (1978) Arch. Pathol. Lab. Med. 102:113.


Cytokeratin Pan

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Host / Source









Human Cytokeratin Pan


0, 1 ml



Storage Temperature

2-8 °C/-20 °C

Shipping Temperature

2-8 °C


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