Mouse anti-Human Kappa (unconjugated), Clone KP-53, IgG1, Purified

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/500
Immunohistology-paraffin Yes 1/500
Immunohistology-resin Not tested
ELISA Yes 1/10000
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

Proteolytic digestion prior to staining of paraffin sections with Pronase (LINARIS CatNo. E110) increase
the staining sensitivity.

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.