Mouse anti-Human Ki-67 (unconjugated), Clone MIB-1, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E059
Category
Quantity
5 ml
Buy at BIOZOL

Background

Der monoklonale Antikörper gegen das Zellproliferationsantigen Ki-67 weist dieses Kernprotein in allen Phasen der mitotischen Zellteilung nach. Stark profilierendes Gewebe zeigt nach der entsprechenden Vorbehandlung eine intensive Kernfärbung.

Normal Tissues

In tissues that are naturally subject to high cell proliferation, such as tonsils or intestinal epithelium, the nuclei of cells that are in an active cell division stage (G1, S, G2 or M phase) are stained.

Abnormal Tissues

In tumor tissues of different origin, such as lymphoma, breast, and lung carcinoma, the cell nuclei of the different cycle phases are stained as in normal tissue. The intensity and frequency of stained nuclei provide a prognostic index for malignant tumors.

Cell nuclei during the resting phase of the cells (G0 phase) are negative.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Ki-67 (MIB-1) (Bottle, 5 ml)

Antigen

Recombinant Ki-67 from E.coli (encoded by 1002bp c-DAN fragment).

Specificity

Human nuclear Ki-67

Abnormal Tissue

Proliferating tumors of different origin

Clone

MIB-1

Isotype

Mouse lgG1

Pretreatment

Pretreatment with HISTOSAFE-ENHANCER (LINARIS CatNo E7000) is required for paraffin sections.

Acetone fixation is recommended for freezing material.

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Tonsil or breast carcinomas

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Gerdes J. Ki-67 and proliferation marker useful for immunhistological diagnostic and prognostic evaluations in human malignancies. Seminars in Cancer Biology 1, 199-206 (1990).
  • Gerdes J., Li L., Schlueter C., Duchrow M., Wohlenberg C., Gerlach C., et al. Immunobiochemical and molecular biologic characterization of the cell proliferation assoziated nuclear antigen that is defined by monoclonal antibody Ki-67. Am. J. Pathol. 138;867-873 (1991)
  • Tungekar M.F., Gatter K.C., Dunnill M.S., and Manson D.Y. Ki-67 immunostaining and survival in operable lung cancer. Histopathol.19; 545-550 (1991)
  • Wintzer H.O., Zipfel I., Schulte-Mönting J., Hellerich U., and von Kleist S. Ki-67 immunostaining in human breast tumors and ist relatuionship to prognosis. Cancer 67; 421-428 (1991)
  • Cattoretti G., Becker M.H., Key G., Duchrow M., Galle J., et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferanting cells in microwave-processed-fixed paraffin sections. J. Pathol. 168; 357-363 (1992)
Specificity

Ki-67
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

MIB-1
Antigen

Human Ki-67
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet