Mouse anti-Human LCA (Leucocyte Common Antigen) (unconjugated), Clone 2B11+PD7-26, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E005
Category
Quantity
6 ml
Buy at BIOZOL

Background

Leukocyte common antigen (LCA) or T 200 glycoprotein was originally discovered in mice. It is composed of a family of proteins with high molecular weights (MG 180, 190, 205, 220 kD).

T 200 glycoprotein is found exclusively on cells of the hematopoietic system and is widely distributed within this system. This marker is expressed by T and B lymphocytes as well as myeloid cells and their progenitors in different variations.

A structural homolog of the mouse T 200 glycoprotein was identified in the human body. Again, all cells of the hematopoietic system expressed it. It was not found in non-hematopoietic tissues.

Synonyms

T 200 (CD45/CD45RB), LCA, Leucocyte Common Antigen T200

Reagents sufficient for about 60-120 tissue sections

  • 1 dropper bottle HISTOPRIME® Leucocyte marker (Bottle, 6 ml)

Antigen

Antigen, T 200 (CD45/CD45RB)

Specificity

Lymphoid cells

Abnormal Tissue

Differentiation into non-hematopoietic, undifferentiated neoplasms and malignant lymphomas

Clone

PD7/26 and 2B11

Isotype

Mouse lgG1

Fusion Partners

Female Balb/ c mice were immunized with human pheoyhromocytoma tissue. Spleen cells from these animals were then fused with NS-1 myeloma cells using the method of Galfre et al.³.

The antibody reagent E005 contains two monoclonal antibodies (clones PD7/26 and 2B11). Both antibodies show a reaction on cells of the hematopoietic system only in connection with the T 200/LCA glycoprotein. At the same time, epitopes in the CD45RB cluster are also detected. Immunohistochemically, the cell surface stains intensely, while the cytoplasmic reaction is less intense. No reaction occurs in non-hematopoietic tissue (e.g. epithelia or connective tissue).

Furthermore, with the monoclonal antibodies a differentiation of malignant lymphomas from non-hematopoietic, undifferentiated neoplasms is possible.

Pretreatment

Proteolytic pretreatment with enzymes not required.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections

Control Tissue

AppLymph nodes, thymus, spleen

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-A5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Warner R.A., Gatter K.C., Falini B. et al.: Diagnosis of human lymphoma with monoclonal and leucocyte antibodies. N. Engl. J. Med. 309; 1275-1281 (1983)
  • Michie S.A., Spagnolo D. V., Dunn K.A., Warnke R. A., and Rouse R.V.: A panel approach to the evaluation of the sensitivity and specificity of antibodies for the diagnosis of routinly processed histologically undifferentiated human neoplasms. Am J. Clin.Pathol. 88; 457-462 (1987)
  • Streuli M., Morimoto C., Schreiber M., Schlossmann S.F., Saito H.: Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leucocyte common antigens. J.Immunol. 141; 3910-3914 (1988)
  • Schraven B., Roux M., Hutmacher B., and Meuer S. C.: Alternative pathway activation of human T-lymphocytes involves the CD45 glycoproteins. N7.4 pp 640-643 In: Leucocyte Typing IV; White cell differntiation antigens. W.Knapp et al. eds. Oxford University Press (1989)
Specificity

LCA (Leucocyte Common Antigen)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

2B11+PD7-26
Antigen

Human LCA (Leucocyte Common Antigen)
Quantity

6 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet