Mouse anti-Human p170-Glycoprotein (MDR) (unconjugated), Clone JSB-1, IgG1, S/N

Concentrated Antibody for Immunohistochemistry

SKU:
K035
Category
Quantity
0, 5 ml
Buy at BIOZOL

Background

The antibody JSB-1 reacts with a conserved cytoplasmic epitope of the plasma membraneassociated 170 – 180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples.

Volume/Quantity

1 ml

Product Form

Tissue culture supernatant – liquid

Approx. Protein Concentrations

IgG concentration 250 µg/ml

Preservatives Stabilisers

0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin (BSA)

Immunogen

P-170 Glycoprotein

Clone Number

JSB-1

Isotype

IgG1 (Mouse)

Fusion Partners

Hybridoma cell line obtained by fusion of lymph node cells from immunized mouse (inbred Balb/C strain) with SP2/O mouse myeloma cells.

Suggested Working Dilution

FlowCytometry Yes
Immunohistology-frozen Yes 1/20-1/40
Immunohistology-paraffin Yes 1/20-1/40
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG FITC conjugate – (LINARIS CatNo LST0009B)
F(ab‘)2 rabbit anti-mouse IgG R-PE conjugate – (LINARIS CatNo LST0012A/B)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Immunohistology

Pre-treatment

This product requires protein digestion e. g. Pronase (LINARIS CatNo E110) or heat treatment with HistoSafe Enhancer (LINARIS CatNo E7000) prior to staining of paraffin sections.

Positive Control Tissue

Liver, kidney, colon, capsule, pancreas, lung.

Recommended Secondary Reagents

VECTASTAIN-ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
VECTASTAIN-ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC1209)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Scheper, R.J. et al. (1986). Int. J. Cancer 42: 389.

Dalton, W.S.et al. (1989). J. Clin. Oncol. 7: 415.

Tiirikainen, M. et al. (1992). Ann. Haematol. 65: 124.

Grogan, T. et al. (1993). Blood 81: 490.

Itsubo, M. et al. (1994). Cancer 73: 298.

Toth, K. et al. (1994). Am. J. Pathol. 144: 227

Gala et al. (1994). J. Clin. Pathol. 47: 619.

Sinicrope et al. (1994). Cancer 74: 2908.

Balaini et al. (1995). New Engl. J. Med. 333: 1380

Chevillard et al. (1996). Cancer 77: 292

Moran et al. (1997). Eur. J. Cancer 33: 652.

Specificity

p170-Glycoprotein (MDR)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

JSB-1
Antigen

Human p170-Glycoprotein (MDR)
Quantity

0, 5 ml
Format

Supernatant
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet