Mouse anti-Human PAP (Prostatic Acid Phosphatase) (unconjugated), Clone PAP29, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E043
Category
Quantity
5 ml
Buy at BIOZOL

Background

Human prostate-specific acid phosphatase (PAP, EC 3.1.2.2) is an isoenzyme of acid phosphatase and catalyzes the hydrolysis of phosphoric acid esters. PAP is present in large amounts in prostatic gland tissue and seminal fluid. Other isoforms of the enzyme are found in erythrocytes, leukocytes, kidneys, bone, spleen, pancreas, and liver.
PAP is an enzyme with a molecular weight of approximately 100 kDa, consisting of two identical sub-enzymes of 48 kDa. PAP is present in large amounts in the epithelial cells of the prostate gland and its excretory ducts. In smaller amounts, PAP also appears to be present in pancreatic islets, parietal cells of the stomach, renal tubules, ureteral epithelium, and male anal glands and scattered cells in the rectum (2,3).
The enzyme is also detected in benign and malignant prostate tumors.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® PAP (Bottle, 5 ml)

Antigen

Purified Prostate Specific Acid Phosphatase (PAP)

Specificity

PAP expressing cells

Abnormal Tissue

Primary and metastatic neoplasms of the prostate gland

Clone

PAP-29

Isotype

Mouse lgG2a

Fusion Partners

BALB/C mice were immunized with purified human prostate-specific acid phosphatase. Spleen cells from these animals were fused with mouse myeloma cells. The resulting hybridoma cells were used to obtain ascites in mice.

The monoclonal antibody E043 (PAP-29) selectively recognizes prostate-specific acid phosphatase. It reacts with acid phosphatase-expressing normal prostate epithelial cells as well as with their primary and metastatic neoplasms. However, the antibody does not react with prostate specific antigen (PSA). It makes sense to use an antibody against PSA (e.g. LINARIS CatNo E040) in parallel for the detection of prostate tumors and their metastases.

Pretreatment

Pretreatment with proteolytic enzymes not required

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Prostate

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Luchter-Wasyl, E. and Ostrowski, W. (1974) Biochim. Biophys. Acta 265; 349ff.
  • Hains, A. et al. (1989) Br. J. Cancer 60; 887ff.
  • Kamoshida, S. and Tsutsumi Y. (1990) Hum. Pathol. 21; 1108ff.
Specificity

PAP (Prostatic Acid Phosphatase)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

PAP29
Antigen

Human PAP (Prostatic Acid Phosphatase)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet