Mouse anti-Human von Willebrand Factor (unconjugated), Clone F8/86, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K033
Category
Quantity
0, 1 mg
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Background

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi’s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Volume/Quantity

0.1 mg

Product Form

Purified IgG – liquid

Buffer

Phosphate buffered saline with 0.1 mg/ml BSA

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Preservatives Stabilisers

0.05 Sodium Azide (NaN3)

Specificity

The monoclonal antibody against Von Willebrand factor K033 is used to stain (cytoplasmic, granular) endothelial cells and megakaryocytes in human tissue. Tumors originating from endothelial cells or megakaryocytes can be deployed by detecting this antigen. In addition, vascularization within different tissues can be assessed and tumor invasion into blood vessels can also be detected

Immunogen

Human plasma vWF protein was used as the immunogen for this von Willebrand Factor antibody.

Clone Number

F8/86

Isotype

IgG1 (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 0.1-0.2 µg/ml
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Tonsil

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

von Willebrand Factor
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

F8/86
Antigen

Human von Willebrand Factor
Quantity

0, 1 mg
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet