Mouse anti-Vimentin (unconjugated), Clone V9, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K034
Category
Quantity
0, 1 ml
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Background

K034 recognises vimentin, a 57kD molecule that forms part of the cytoskeleton of non-epithelial cells. It is expressed by a range of cell types including smooth muscle cells from blood vessel walls, astrocytes and various cell lines. The antibody may be used to recognise tumours of mesenchymal origin such as sarcoma and lymphoma.

Clone V9 is reported as being suitable for use in Western blotting (6).

Volume/Quantity

0.1 ml

Product Form

Ascites fluid – liquid

Preservatives Stabilisers

15 mM Sodium Azide (NaN3)

Immunogen

Vimentin purified from porcine eye lens

Clone Number

V9

Isotype

IgG1 (Mouse)

Fusion Partners

Spleen cells of immunised BALB/c mice were fused with cells from a mouse myeloma cell line.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/500 – 1/750
Immunohistology-paraffin Yes 1/500 – 1/750

Immunofluorescence Yes 1/40
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes 1/400
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Westernblotting

Chemiluminescent Substrate

DuoLuX Chemiluminescent Substrate for Alkaline Phosphatase (AP) (LINARIS CatNo ESA6605) and
Peroxidase (POD) (LINARIS CatNo ESP6604).

Western Blotting Immunodetection Kit

ABC-AmP Chromogenic Detection Kit anti-Mouse IgG (LINARIS CatNo EDA6402) or anti-Rabbit IgG (LINARIS CatNo EDA6401)

Weight Standard

Biotinylated protein molecular weight marker (LINARIS CatNo RPB1400). Molecular weight range from
19kD to 222kD.

Immunohistology

Positive control

Human skin

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108) or HistoGreen-Substrate for POD (LINARIS CatNo 109)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of reconstitution.

Heid, H.W. et al. (1988) Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37: 137-157.

Joling, P. et al. (1996) Immunohistochemical demonstration of cellular antigens of the cat by antihuman antibodies. Vet. Immunol. Immunopathol. 53: 115-127.

Komuro, T. et al. (1996) Anti-c-Kit protein immunoreactive cells corresponding to the interstitial cells of Cajal in the guinea-pig small intestine. J. Auton. Nerv. Syst. 61: 169 – 174.

Ando, H. et al. (1999) Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity. Am. J. Physiol. 276 (5 Pt 2): H1755 -68.

Josephsen, K. et al. (1999) Selective but nonspecific immunolabeling of enamel protein-associated compartments by a monoclonal antibody against vimentin. J. Histochem. Cytochem. 47 (10): 1237 –
1245.

Sanderson, J. et al. (2000) A human lens model of cortical cataract: Ca2+-induced protein loss, vimentin cleavage and opacification. Invest. Ophthamol. Vis. Sci. 41: 2255 – 2261.

Specificity

Vimentin
Species Reactivity

Bovine, Cat, Chicken, Dog, Guinea Pig, Horse, Human, Monkey, Rabbit, Rat, Swine
Host / Source

Mouse
Isotype

IgG1
Application

IF, IHC-F, IHC-P, WB
Clone

V9
Antigen

Human Vimentin
Quantity

0, 1 ml
Format

Purified
Storage Temperature

2-8 °C / -20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet