HistoSafe Enhancer Unmasking Solution, (04x)-Concentrate
Preatreatment Reagent for unmasking of FFPE-IHC Sections
HISTOSAFE-ENHANCER is a novel technology developed by LINARIS to enhance or enable staining results on formaldehyde-fixed, paraffin-embedded tissue.
Monoclonal antibodies can now be used successfully on paraffin-embedded material when the use of proteases has been unsuccessful. Polyclonal antibodies also often give better results when tissue sections have been pre-treated with HISTOSAFE-ENHANCER.
Formalin fixation can mask epitopes due to cross-linking and make them inaccessible for antibody binding. Treatment with HISTOSAFE-ENHANCER breaks up these crosslinks. As a result, both intracellular and membrane antigenic determinants are unmasked and made accessible to antibodies.
This means that numerous antibodies that were previously not paraffin-embedded can now be used routinely on fixed kerosene sections for the first time.
With the aid of the enhancer solution, it is possible to visualize antigens in the test material without the use of proteolytic enzymes. As a rule, treatment with HISTOSAFE-ENHANCER produces better results than pretreatment with citrate buffer in the microwave.