Mouse anti-Human Actin Alpha (Sarkomeric) (unconjugated), Clone 5C5, IgM, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K045
Category
Quantity
0, 1 ml
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Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure.

Volume/Quantity

0.1 m

Product Form

Ascites – liquid

Preservatives Stabilisers

0.09 Sodium Azide (NaN3)

Immunogen

BALB/C mice were injected with purified rabbit striated muscle actin

Clone Number

5C5

Isotype

IgM (Mouse)

Specificity

Monoclonal anti-ALPHA-sarcomeric actin has been used as a marker for rhabdomyosarcoma. This reacts with a-skeletal and a-cardiac muscle actins. It does not react with smooth muscle tissue.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/50-1/100
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tisssue

Skeletal and cardiac muscles

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Actin Alpha (Sarkomeric)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgM
Application

IHC-F, IHC-P
Clone

5C5
Antigen

Human Actin Alpha (Sarkomeric)
Quantity

0, 1 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet