Mouse anti-Human Actin Alpha (Sarkomeric) (unconjugated), Clone 5C5, IgM, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E045
Category
Quantity
5 ml
Buy at BIOZOL

Background

Actin and myosin represent the two major cytoskeletal proteins responsible for cell motility. Besides muscle contraction, these proteins are also involved in many other movement processes of single cells and organs, e.g. locomotion, secretion, phagocytosis, cell division, etc. Fibrous actin molecules form thin filaments in the contractile system of cells. Six electrophoretically characterized isoforms are found in muscle cells. Smooth and striated muscle have different isoforms of alpha-actin. Detection of sarcomeric or smooth muscle actin can also be informative in cell differentiation of tumors.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® a-Actin, sarcomeric (Bottle, 5 ml)

Antigen

Sarcomeric alpha-actin from rabbit muscle

Specificity

Skeletal and cardiac muscles

Abnormal Tissue

Rhabdomyosarkome

Clone

5C5

Isotype

Mouse lgM

Fusion Partners

Spleen cells from immunized mice were fused with mouse myeloma cells. Purified rabbit sarcomeric actin was used as immunogen. Anti-actin producing hybridoma cells were further used for ascites recovery.

The monoclonal antibody E045 (5C5) recognizes sarcomeric alpha-actin (alpha-sr-1) of striated skeletal and cardiac muscles. The antibody shows high reactivity with human actin as well as with actin from sheep, cattle, rabbit, rat and guinea pig. However, it does not react with smooth muscle, for the detection of which we refer to our LINARISCatNo. E046. Antibody E045 can be used to characterize rhabdomyosarcomas.

E045 recognizes actin from fish, frog, rabbit, guinea pig, rat, cattle, sheep, snake, etc. in addition to sarcomeric human actin.

Pretreatment

Pretreatment with proteolytic enzymes not required.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Skeletal and cardiac muscles

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Schurch, W. et al. Amer. J.Pathol. 128, 91 (1987)
  • Babai, F. et al. Virchow Arch. B. 55, 263 (1987)
  • Skalli, O. er al. Amer. J. Pathol. 130, 515 (1988)
Specificity

Actin Alpha (Sarkomeric)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgM
Application

IHC-F, IHC-P
Clone

5C5
Antigen

Human Actin Alpha (Sarkomeric)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet