Mouse anti-Human Actin Alpha (Smooth Muscle) (unconjugated), Clone 1A4, IgG2a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E046
Category
Quantity
5 ml
Buy at BIOZOL

Background

Actin and myosin represent the two major cytoskeletal proteins responsible for cell motility. In addition to muscle contraction, these proteins are also involved in many other movement processes of single cells and organs, e.g. locomotion, secretion, phagocytosis, cell division, etc.
Fibrous actin molecules form thin filaments in the contractile system of cells. Six electrophoretically characterized isoforms of actin can be distinguished in mammals. Four of these isoforms are found in muscle cells. Smooth and transversely striated muscle have different isoforms of alpha-actin. Detection of sarcomeric or smooth muscle actin can also be informative in the differentiation of muscle cells and in the differentiation or dedifferentiation of tumors.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® a-Actin, smooth muscle (Bottle, 5 ml)

Antigen

Smooth muscle alpha-actin(1-10) synthetic

Specificity

Smooth muscle and smooth muscle tumors in human tissue.

Clone

1A4

Isotype

Mouse lgG2a

Fusion Partners

Spleen cells from immunized mice were fused with mouse myeloma cells. N-terminal decapeptide of smooth muscle alpha-actin was used as immunogen. Anti-actin producing hybridoma cells were further used for ascites recovery.

The monoclonal antibody E046 (1A4) recognizes smooth muscle alpha-actin. It does not react with striated muscle, for the detection of which we refer to our LINARIS CatNo E045. Antibody E046 can be used to characterize smooth muscle organs and tumors. Also a differentiation of fibroblast actin in tissue cultures is possible with this antibody.

Pretreatment

Pretreatment with proteolytic enzymes not required.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Smooth muscles

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Schurch, W. et al. Amer. J. Pathol. 128, 91 (1987)
  • Babai, F. et al. Virchow Arch. B. 55, 263 (1988)
  • Skalli, O. et al. Amer. J. Pathol. 130, 515 (1988)
Specificity

Actin Alpha (Smooth Muscle)
Species Reactivity

Bovine, Chicken, Dog, Frog, Goat, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep, Snake, Swine
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

1A4
Antigen

Human Actin Alpha (Smooth Muscle)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet