Mouse anti-Human Alpha Fetoprotein (AFP) (unconjugated), Clone A-013-01, IgG1a, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E042
Category
Quantity
5 ml
Buy at BIOZOL

Background

Alpha-fetoprotein (AFP) is a fetal globulin with a molecular weight of approximately 70 kDa. AFP is synthesized in fetuses in relatively high concentrations in the embryonic yolk sac, gastrointestinal tract and liver. During normal pregnancy, it is detectable in both maternal and fetal serum. Shortly after birth, AFP levels drop to a very low level (20ng/ml).
Increasing AFP levels in adults (outside of pregnancy) are usually associated with hepatocellular carcinoma or yolk sac tumor (testicular or pineal organ). The increasing AFP synthesis rate of malignant hepatic cells is interpreted as a return of the cells to the more active fetal stage.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® AFP (Bottle, 5 ml)

Antigen

Purified alpha-fetoprotein from umbilical cord blood

Specificity

Human alpha-fetoprotein
No cross-reaction with HSA, CEA, HCG, ferritin and PSA

Abnormal Tissue

Primary hepatocarcinoma, yolk sac tumors.

Clone

F1-6P2A8-P2B9A9

Isotype

Mouse lgG1

Fusion Partners

Spleen cells from immunized mice were fused with mouse myeloma cells (P3X63-Ag8,653).
Purification of the isolated monoclonal antibodies was performed by DEAE chromatography.

The AFP monoclonal antibody E042 recognizes the antigenic determinants of the protein both in the fetal yolk sac and in fetal liver, and during malignant transformation in liver tissue. AFP detection in the liver can be considered a marker of hepatocellular carcinoma. In tumors of the testis, it may serve to differentiate them from seminomas, which usually do not express AFP.

Pretreatment

Pretreatment with proteolytic enzymes not required

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Fetal liver or yolk sac

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100)
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Mann K., Lamerz R., Löhrs U., Nathraht W., Rattenhuber U., und H. J. Karl (1980) HCG und AFP bei Keimzelltumoren des Hodens: Vergleich von Serum- und Gewebsbefunden. In: CEA und andere Tumormarker, Ein Symposiumband. Uhlenbruck und Wintzer Herausg. TumorDiagnostik Verlag (Leonberg) S. 379-388
  • B. Norgaard-Pedersen (1980) Die klinische Anwendung von Alpha-Foetoprotein und Human Choriongonadotropin in germinalen Zelltumoren. In: CEA und andere Tumormarker, Ein Symposiumband. Uhlenbruck und Wintzer Herausg. TumorDiagnostik Verlag (Leonberg) Abstract S.389.
Specificity

Alpha Fetoprotein (AFP)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1a
Application

IHC-F, IHC-P
Clone

A-013-01
Antigen

Human Alpha Fetoprotein (AFP)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet