Mouse anti-Human CEA (unconjugated), Clone 85A12, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E056
Category
Quantity
6 ml
Buy at BIOZOL

Background

CEA is a phospho-gycoprotein and a component of the glycocalix of the embryonic endodermal epithelium. It is a member of the immunoglobulin superfamily and the CD66 molecules (MW 170 to 200 kDa) expressed by neutrophilic granulocytes. CEA is present in cell extracts of many carcinoma types, especially intestinal tumors. In serum, CEA is determined as a tumor marker whose concentration is reported to correlate well with tumor mass. CEA expression is usually a sign of de-differentiation in gastric and intestinal carcinomas.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® CEA (Bottle, 5 ml)

Antigen

Human Carcinoembryonic Antigen

Specificity

CEA

Abnormal Tissue

Colon and gastric carcinoma

Clone

85A12

Isotype

Mouse lgG1

Fusion Partners

Spleen cells from immunized animals were fused with mouse myeloma cells. Purified CEA served as immunogen. Anti-CEA antibody-producing cell lines were used to obtain ascites. The antibody is available in purified form.

The monoclonal antibody against CEA (LINARIS CatNo E056, clone 85A12), labels normal colon tissue only weakly due to the low CEA synthesis rate. The same is true for benign epithelial tumors such as serous and mucinous cystadenomas. In contrast, the antibody has a strong affinity for tumor cells from adenocarcinomas of the colon. Furthermore, CEA expression is found in medullary thyroid carcinomas, pancreatic ductal carcinomas, and adenocarcinomas of the cervix, ovary, lung, and mammary glands. Endometrial carcinomas are usually CEA negative.

Pretreatment

Pre-treatment of formaldehyde-fixed material with proteolytic enzymes (LINARIS Cat. No. E110) not required, but improves the results of the staining just as much as pre-treatment with HISTOSAFE-Enhancer (LINARIS Cat. No. E7000)!

Incubation Period

1 hour at room temperature a

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Colon Cancer

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Ghosh, A. K. et al. (1987) Immunological staining reactions of anti-CEA and anti-HMFG monoclonal antibodies on benign and malignant exfoliated mesothelial cells. J. Clin. Path. 40; 1424-1427
  • Tron, V. et al. (1987) CEA and HMFG staining of malignant mesothelioma and adenocarcinoma of the lung. Arch. Pathol. Lab. Med.111; 291-293
  • MacConald, F. et al. (1988) Expression of CEA, Ca1225, Ca 19-999 and HMFG in ovarian tumors. J. Clin. Path. 41; 260-264
  • Potterr, C. R. et al. (1988) CEA and HMFG in hyperplastic and malignant lesions of the breast. Pathol. Res. Pract. 183; 271-276.
Specificity

CEA
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

85A12
Antigen

Human CEA
Quantity

6 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet