Mouse anti-Human Choriongonadotropin (ß-HCG) (unconjugated), Clone SC1, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K009
Category
Quantity
0, 5 mg

Background

hCG is a 40 kDa glycoprotein composed of two subunits, α and β. The α subunit is common for hCG, LH, follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the β subunit is unique for hCG although its amino acid sequence partially is identical with that of the β subunit of LH. hCG is the most important marker of gestational trophoblastic cells, being present in syncytiotrophoblastic cells and cells of the intermediate trophoblast but absent in cytotrophoblasts. hCG expression has also been demonstrated in some non-trophoblastic tumours, e.g. in 14% of patients with hepatocellular carcinoma.
In pregnancy, the serum hCG concentration increases at an exponential rate initially, then peaks at around 100 kIU/L at about the tentafter conception. The concentration decreases about ten fold over the next several weeks followed by a small increase near term.

Synonyms

Choriongonadotropin, hCG Beta, Chorionic Gonadotropin Beta

Volume/Quantity

0.5 mg

Product Form

Purified IgG – liquid

Clone Number

SC1

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer

Phosphate buffered saline pH7.0

Approx. Protein Concentrations

IgG concentration 6,73 mg/ml

Preservatives Stabilisers

0.02% Sodium Azide (NaN3)

Immunogen

Human Chorionic Gonadotropin (hCG)

Isotype

IgG1 (Mouse)

Specificity

Intact hCG 100%
Beta-hCG 100%
Alpha-hCG 0.0%
LH 0.3%
TSH 0.2%
FSH 0.8%

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/500 – 1/1000
Immunohistology-paraffin Yes 1/500 – 1/1000
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Placenta

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108) or HistoGreen-Substrate for POD (LINARIS CatNo E109)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

ß-HCG (Choriongonadotropin)

Species Reactivity

Human

Host / Source

Mouse

Isotype

IgG1

Application

IHC-F, IHC-P

Clone

SC1

Antigen

Human ß-HCG (Choriongonadotropin)

Quantity

0, 5 mg

Format

Purified

Storage Temperature

2-8 °C / -20 °C

Shipping Temperature

2-8 °C

Documents

pdf Datasheet