Mouse anti-Human Collagen Type-IV (unconjugated), Clone COL94, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K047
Category
Quantity
0, 1 ml
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Background

The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different α chain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain.
Monoclonal Anti-Collagen Type IV (mouse IgG1 isotype) is derived from the COL-94 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen Type IV.

Volume/Quantity

0.1 ml

Product Form

Ascites – liquid

Preservatives Stabilisers

15 mM Sodium Azide (NaN3)

Immunogen

Purified full length native human Collagen IV.

Clone Number

COL94

Isotype

IgG1 (Mouse)

Specificity

The monoclonal antibody E047 (COL-94) is directed exclusively against type IV collagen. It does not react with other types of collagen or with other connective tissue proteins. The anticollagen IV antibody allows to reveal the importance of basement membranes in tissue differentiation, cell growth or neoplasia. Destruction of the epithelial basement membrane is considered an important criterion for malignant invasive growth. In particular, invasive ductal breast carcinomas usually show complete loss of the basement membrane.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/100-1/500
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Human skin tissue.

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Collagen Type-IV
Species Reactivity

Human, Monkey
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

COL94
Antigen

Human Collagen Type-IV
Quantity

0, 1 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet