Mouse anti-Human Collagen Type-IV (unconjugated), Clone COL94, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E047
Category
Quantity
5 ml
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Background

Connective tissues are composed of extracellular, structural, and supportive elements that perform multiple functions in tissue differentiation and formation. A major component of connective tissue, as well as adjacent basement membranes, is the fibrous protein collagen, which occurs in various forms. Basement membranes mainly have collagen type IV collagen.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Collagen IV (Bottle, 5 ml)

Antigen

Human Collagen IV

Specificity

Collagen IV alpha and/or alpha2 chain in connective tissue, basement membranes.

Clone

COL-94

Isotype

Mouse lgG1

Fusion Partners

Balb/C mice were immunized with human collagen IV. Spleen cells from these animals were fused with mouse myeloma cells. The resulting hybridoma cells were used to produce ascites. Ascites was processed with stabilizing buffer to obtain a ready-to-use product.

The monoclonal antibody E047 (COL-94) is directed exclusively against type IV collagen. It does not react with other types of collagen or with other connective tissue proteins. The anticollagen IV antibody allows to reveal the importance of basement membranes in tissue differentiation, cell growth or neoplasia. Destruction of the epithelial basement membrane is considered an important criterion for malignant invasive growth. In particular, invasive ductal breast carcinomas usually show complete loss of the basement membrane.
The E047 antibody also reacts with monkey collagen IV, but not with chicken, cat, rabbit, rat, sheep and goat collagen.

Pretreatment

Proteolytic pretreatment with Pronase (LINARIS CatNo E110) for 10 minutes at room temperature or pretreatment on kerosene sections with HistoSafe enhancer (LINARIS CatNo E7000) for 20 minutes in a water bath at 96-100 °C.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Connective tissue

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Barsky S. H., Siegal G. P., Jannotta F., and Liotta L. A. (1983). Loss of basement membrane components by invasive tumors but not by benign counterparts. Lab. Invest. 49; 140-147.
  • Remberger K. and Nerlich A. (1985). Diagnostischer Wert der Darstellung von Basalmembranproteinen in benignen und malignen Mammaveränderungen. Verh. Dtsch. Ges. Pathol. 69; 123-130.
  • Josji M. G., Lee A. K. C., Pedersen C. A. Schnitt S., Camus M. G., and Huges K. S. (1996) The role of immunocytochemical diagnosis of proliferative and neoplastic lesions of the breast. Mod. Pathol. 9; 57-62.
  • Nerlich A. G., (1998). Wert der Basalmembrandarstellung in der Diagnostik invasiver Karzinome Pathologe 19; 89-94.
Specificity

Collagen Type-IV
Species Reactivity

Human, Monkey
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

COL94
Antigen

Human Collagen Type-IV
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet