Mouse anti-Human Cytokeratin-13 (CK13) (unconjugated), Clone Ks13.1, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K018
Category
Quantity
0, 05 mg
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Background

Cytokeratins are a group of 20 different water-insoluble proteins. Together with microfilaments and microtubules, they constitute the cytoskeleton of epithelial cells. It is usually the case that different cytokeratins are found simultaneously in a cell. This is independent of cell type, cell environment, disease and differentiation stage. If a conversion from normal to neoplastic cells takes place, the expression of these proteins is maintained.

Synonyms

Cytokeratin 13

Volume/Quantity

50 µg

Product Form

Purified IgG – lyophilised

Reconstitution

Reconstituted with 1 ml distilled water

Clone Number

Ks 13.1

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer

Phosphate buffered saline

Approx. Protein Concentrations

IgG concentration 50 µg/ml

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin (BSA)

Immunogen

Cytokeratin 13 purified from human oesophagus

Isotype

IgG1 (Mouse)

Specificity

This antibody reacts with human cytokeratin 13 with minor affinity for cytokeratins 14 and 17.
Cytokeratin 13 (54 kD) is expressed by several stratified squamous epithelia such as vagina, tongue, oral mucosa, oesophagus, epidermis and urothelium of bladder.

cervical carcinomas, cloacogenic carcinomas and transitional cell carcinoma of bladder also express cytokeratin 13.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/50
Immunohistology-paraffin Yes 1/50
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections with
HistoSave-Enhancer. (LINARIS CatNo E7000) or protein digestion pre-treatment of paraffin sections e.
g. Pronase (LINARIS CatNo E110).

Recommended Secondary Reagents

ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo MAK928)

Westernblotting

Chemiluminescent Substrate

DuoLuX Chemiluminescent Substrate (LINARIS CatNo ESC6600) for Alkaline Phosphatase (AP) and Peroxidase (POD).

Western Blotting Immunodetection Kit

Vectastain ABC-AmP Chromogenic Detection Kit anti-Mouse IgG (LINARIS CatNo EDA6402) or anti-Rabbit IgG (LINARIS CatNo EDA6401)

Weight Standard

Biotinylated protein molecular weight marker (LINARIS CatNo ESP1400). Molecular weight range from 19kD to 222kD.

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Moll, R. et al. (1988). Cytokeratins in normal and malignant transitional epithelium. Am. J. Pathol. 132, 123-144.

Franke, W. W. et al. (1986). Cell typing of epithelia and carcinomas of the female genital tract using cytoskeletal proteins as markers. Banbury Report 21: Viral Etiology of Cervical Cancer, Cold Spring Harbor Laboratory (NY), 121-148.

Moll, R. et al. (1982). The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24.

Specificity

Cytokeratin-13 (CK13)
Species Reactivity

Bovine, Human, Mouse, Rat
Host / Source

Mouse
Isotype

IgG1
Application

IF, IHC-F, IHC-P, W
Clone

Ks13.1
Antigen

Human Cytokeratin-13 (CK13)
Quantity

0, 05 mg
Format

Purified
Storage Temperature

2-8 °C / -20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet