Mouse anti-Human Cytokeratin-18 (CK18) (unconjugated), Clone Cy90, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E041
Category
Quantity
5 ml
Buy at BIOZOL

Background

Epithelial cells and their derivatives typically consist of intermediate filaments composed of approximately 20 different related polypeptides. The molecular weights of these cytokeratins range from 40,000 to 69,000 Da. Each epithelial tissue shows a specific and stable expression pattern of these cytokeratin subunits.

Tumors originating from epithelia retain the cytokeratin expressions of normal epithelial cells.
For this reason, carcinomas can be identified and classified using antibodies that specifically react with the various cytokeratins.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin 18 (Bottle, 5 ml)

Antigen

Human Cytokeratin 18 Peptide

Specificity

Reacts specifically with human cytokeratin 18

Abnormal Tissue

Carcinomas of simple epithelia (such as intestinal, bronchial, urinary tract, liver, and glandular epithelium).

Clone

CY-90

Isotype

Mouse lgG1

Fusion Partners

Spleen cells from immunized mice were fused with mouse myeloma cells. Epithelioma (A-431) and mammary carcinoma (MCF-7) cells were used as immunogens. Antibody-producing hybridoma cells were used for ascites retrieval.

The monoclonal antibody E041 (CY-90) reacts highly specifically with human cytokeratin18 in a variety of simple epithelia (e.g. with intestinal, bronchial, urinary tract, liver and glandular epithelia of prostate and uterus).
It does not react with squamous epithelia of the following organs: epidermis, hair follicles, sebaceous glands, tongue, esophagus and vagina or with non-epithelial cells.

Pretreatment

Proteolytic pretreatment with 0.1% Pronase (Art. No. E110), 10 min at room temperature or HISTOSAFE-ENHANCER (Art. No. E7000) 10 min at 96-100°C

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Colon carcinoma or appendix

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Moll, R. Franke W. W., Schiller, D. L., Geiger, B. and Krepler, R. The catalog of human cytokeratin polypeptides: Pattern of expression of specific cytokeratins in normal epithelia, tumors and cultured cells. Cell 31; 11-24 (1982)
Specificity

Cytokeratin-18 (CK18)
Species Reactivity

Bovine, Human, Rat
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

Cy90
Antigen

Human Cytokeratin-18 (CK18)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet