Mouse anti-Human Cytokeratin-7 (CK7) (unconjugated), Clone OV-TL 12/30, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K061
Category
Quantity
0, 5 ml
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Background

Anti-cytokeratin 7 reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression. CK 7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas. Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms.
This antibody does not recognize intermediate filament proteins.

Volume/Quantity

0.5 mg

Product Form

Supernatant – liquid

Buffer

Tris Buffer pH 7.3 – 7.7 with 1% BSA

Preservatives Stabilisers

0.09 Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 1 mg/ml


Immunogen

Human plasma vWF protein was used as the immunogen for this von Willebrand Factor antibody.

Clone Number

OV-TL 12/30

Isotype

IgG1 (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/100-1/500
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Colon adenocarcinoma

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Cytokeratin-7 (CK7)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

OV-TL 12/30
Antigen

Human Cytokeratin-7 (CK7)
Quantity

0, 5 ml
Format

Purified
Storage Temperature

-20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet