Mouse anti-Human Cytokeratin Breitband (CK Pan) (unconjugated), Clone AE1+AE3, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

6 ml


A group of 19 different water-insoluble proteins forms the cytokeratins. Together with microtubules and microfilaments, they form the cytoskeleton of epithelial cells. Normally, it is the case that different cytokeratins are found simultaneously in one cell. This is independent of cell type, cell environment, disease and differentiation stage.

If a conversion from normal to neoplastic cells takes place, the expression of these proteins is maintained.


Cytokeratin Pan

Reagents sufficient for about 60-120 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin Pan (Bottle, 6 ml)


Human epidermal cytokeratin


Epidermal basal layer (AE1)

Abnormal Tissue

Differentiation into carcinoma – non-carcinoma




Mouse lgG1

Fusion Partners

Balb/c mice were immunized with human epithelial keratins and their spleen cells were fused with P3 myeloma cells. Furthermore, these spleen cells were tested for antikeratin activity by ELISA. Antibodies were purified by fractional salt precipitation.

The monoclonal antibody AE1 allows specific recognition of the cytokeratin of the acidic subfamily (40, 48, 50 and 56.5 kD). The monoclonal antibody AE3 has the property to react with all cytokeratin of the basic subfamily. If the two clones are now mixed, a broad reaction spectrum is obtained for a large number of epithelia. Accordingly, this mixture recognizes epithelial neoplasms. There is no known cross-reactivity with other cytoskeletal proteins. Antibodies can also be used to differentiate between carcinoma and non-carcinoma.

Some carcinomas (e.g. urothelial carcinoma) predominantly express cytokeratin 13. Therefore, we recommend our Cytokeratin Cocktail (Cat.No. E020) or a Cytokeratin 13 specific antibody (Cat.No. E018) for this diagnosis. The Cytokeratin Cocktail contains the clones AE1 and AE3 as well as the clone Ks13.1 and therefore allows an even broader application than E006. E006 reacts specifically with cytokeratin 1-8, 10, 14-16 and 19.


Proteolytic pretreatment with 0.1% Pronase (Cat.No.E110) 10 min at room temperature or HISTOPRIME-ENHANCER (Art.No.E7000) 10 min at 96-100°C in a water bath or in a microwave oven

Incubation Period

1 hour by room temperature


Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Appendix or colon carcinoma

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Sun,T.-T and H.Green: Immunofluorescent staining of keratin fibers in cultured cells. Cell 14, 468(1978)
  • Franke, W.W., E.Schmid, M.Osborn and K.Weber: Different intermediate-sized filaments distinguished by immunofluorescence mocroscopy. Proc. Natl. Acad. Sci USA 75,5034-5038 (1978)
  • Sun, T.-T, C. Shih and H. Green: Keratin cytoskeletons in epithelial cells of internal organs. PNAS 76,2813-2817 (1979)
  • Franke, W. W., O. Appelhans, E. Schmid, C. Freudenstein, M. Osborn and K. Weber: Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation 15,7-25 (1979)
  • Sun, T.-T., R. Eichner, A. Schermer, D. Cooper, W. G. Nelson and R. A. Weiss: Classification expression and possible mechanisms of evolution of mammalian epithelial keratins: a unifing model. In: The Cancer Cell Vol.1,the transformed Phenotype A. Levine, W. Topp, G. Vande Woude and J. D. Watson, eds. Cold Spring Harbour Lab., N.Y. 169-176 (1984)
  • Woodcock-Mitchell, J., R. Eichner, W. G. Nelson and T.-T. Sun: Immunolocalisation of keratin polypeptides in human epidermis using monoclonal antibodies. J. Cell Biol. 95, 580-588 (1982)

Cytokeratin Breitband (CK Pan)

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Host / Source









Human Cytokeratin Breitband (CK Pan)


6 ml



Storage Temperature

2-8 °C

Shipping Temperature

20 °C


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