Mouse anti-Human Cytokeratin Cocktail (CK Cocktail) (unconjugated), Clone AE1/3+Ks13.2, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E020
Category
Quantity
5 ml
Buy at BIOZOL

Background

A group of 19 different water-insoluble proteins forms the cytokeratins. Together with microtubules and microfilaments, they form the cytoskeleton of epithelial cells. Normally, it is the case that different cytokeratins are found simultaneously in one cell. This is independent of cell type, cell environment, disease and differentiation stage. If a conversion from normal to neoplastic cells takes place, the expression of these proteins is maintained.

Synonyms

Cytokeratin

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Cytokeratin Cocktail (Bottle, 5 ml)

Antigen

Human keratin

Specificity

Human epithelial cytokeratins

Abnormal Tissue

Differentiation into carcinoma – non-carcinoma

Clone

AE1, AE3 and Ks13.2

Isotype

Mouse lgG1

Fusion Partners

Balb/c mice were immunized with human epithelial keratins and their spleen cells were fused with P3 myeloma cells. Furthermore, these spleen cells were tested for antikeratin activity by ELISA. Antibodies were purified by fractional salt precipitation.

The antibody cocktail exhibits specific reaction with keratin-containing epithelial cells in tissue sections and cultures. It consists of three clones AE1, AE3 and KS 13.1 and demonstrates a broad spectrum of reaction including epidermis, mucosal epithelia, glandular epithelia, squamous epithelia of the ectocervix and urothelia.
The cocktail can be used to differentiate between carcinoma and non-carcinoma. E020 reacts specifically with cytokeratins 1-8, 10, 13-16 and 19.

Pretreatment

Proteolytic pretreatment with 0.1% Pronase (LINARIS CatNo E110) 10 min at room temperature or HISTOPRIME-ENHANCER (LINARIS CatNr E7000)10 min at 96-100°C in a water bath or in a microwave oven

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Appendix or colon carcinoma

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Sun,T.-T and H.Green:Immunofluorescent staining of keratin fibers in cultured cells.Cell14, 468(1978)
  • Franke,W.W.,E.Schmid,M.Osborn and K.Weber:Different intermediate-sized filaments distinguished by immunofluorescence mocroscopy.Proc.Natl.Acad.sci USA 75,5034-5038 (1978)
  • Moll R.,Franke W.W., Schiller D.L.,Geiger B. and Krepler R.:The catalog of human cytokeratin polypeptides:Pattern of expression of specific cytokeratins in normal eoithelia,tumors and cultured cells. Cell 31, 11-24 (1982)
  • Franke,W.W.,O.Appelhans,E.Schmid,C.Freudenstein,M.Osborn and K.Weber:Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin.Differentiation 15,7-25 (1979)
  • Sun,T.-T.,R.Eichner,A.Schermer,D.Cooper,W.G.Nelson and R.A.Weiss: Classification expression and possible mechanisms of evolution of mammalian epithelial keratins:a unifing model.In:The Cancer Cell Vol.1,the transformed
  • Phenotype.A.Levine,W.Topp,G.Vande Woude and J.D.Watson,eds. Cold Spring Harbour Lab.,N.Y. 169-176 (1984) WoodcockMitchell,J.,R.Eichner,W.G.Nelson and T.-T.Sun:Immunolocalisation of keratin polypeptides in human epidermis using monoclonal antibodies.J.Cell. Biol.95,580-588 (1982)
Specificity

Cytokeratin Cocktail (CK Cocktail)
Species Reactivity

Bovine, Chicken, Human, Mouse, Rabbit, Rat
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

AE1/3+Ks13.2
Antigen

Human Cytokeratin Cocktail (CK Cocktail)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet