Mouse anti-Human Epithelial Membrane Antigen (unconjugated), Clone E29, IgG2a, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K049
Category
Quantity
0, 1 ml
Buy at BIOZOL

Background

Epithelial membrane antigen belongs to a group of homomolecular glycoproteins isolated from the membrane of human milk fat globules. These proteins are also known as human milk fat globulins (HMFG). Their molecular weight ranges from 265 to 400 kDa. In addition to milk, these proteins are found in a number of normal epithelia as well as in tumors derived from them.

Volume/Quantity

0.1 ml

Product Form

Purified IgG – liquid

Buffer

PBS, pH 7.4 with 0.2 % BSA

Approx. Protein Concentrations

IgG concentration 0.2 mg/m

Preservatives Stabilisers

0.09 Sodium Azide (NaN3)

Immunogen

Delipidated extract of human milk fat globule membranes

Clone Number

E29

Isotype

IgG2a (Mouse)

Specificity

The K049 antibody reacts with a variety of human epithelial cells. In epithelial tumors, this marker is also strongly expressed. Thereby, mammary carcinomas show the strongest expression. Moreover, the antibody is particularly reactive in tumors of glandular origin (e.g. sweat glands). The antigen can also be detected in squamous cell carcinomas, even if the epithelium of origin (e.g. epidermis) itself is not reactive. In contrast, non-epithelial tumors do not contain detectable EMA.
The antibody also detects some lymphoid neoplasms, especially CD30-positive large cell lymphomas.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/400-1/800
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Human breast carcinoma

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Epithelial Membrane Antigen
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG2a
Application

IHC-F, IHC-P
Clone

E29
Antigen

Human Epithelial Membrane Antigen
Quantity

0, 1 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet