Mouse anti-Human hCG Beta (Chorionic Gonadotropin Beta) (unconjugated), Clone SC1, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E009
Category
Quantity
6 ml
Buy at BIOZOL

Background

During pregnancy, trophoplasts produce human chorionic gonadotropin (HCG), which is essential for maintaining pregnancy. It is similar to LH in its molecular structure and biological activity. HCG has a molecular weight of about 28000 Da and consists of two subunits: an -chain and a ß-chain. LH, FSH and TSH also have such an -chain. The ß-chain, however, has a different amino acid sequence and is responsible for the specificity of action of the hormone. For this reason, immunological detection of the hormone takes place via the ß-chain. HCG is found in high concentrations in both the blood and urine of pregnant women.

HCG is secreted by trophoblastic neoplasms and chorionic carcinomas, as are, in part, the free ß-chains. However, ß-HCG positive can also occur in non-trophoblastic tumors (e.g. leiomyosarcomas in testis or small intestine). Thus, to detect such tumors, the use of an antibody that specifically detects the ß-chains is useful.

Synonyms

Choriongonadotropin, hCG Beta, Chorionic Gonadotropin Beta

Reagents sufficient for about 60-120 tissue sections

  • 1 dropper bottle HISTOPRIME® ß-HCG (Bottle, 6 ml)

Antigen

human chorionic gonadotropin (HCG)

Specificity

HCG, beta-HCG

Abnormal Tissue

Chorionic carcinomas, trophoblastic neoplasms, germ cell tumors, choriocarcinomatous differentiations of colon, lung, and breast

Clone

SC1

Isotype

Mouse lgG1

Fusion Partners

Balb/c mice were immunized with human chorionic gonadotropin and their splenocytes were fused with mouse myeloma cells P3X63-Ag8.653. Beta-HCG specific antibody producing cell lines were used for ascites recovery. The immunoglobulin fraction was purified column chromatically via DEAE.

E009 detects the ß-chain of HCG in particular, but is also suitable for the detection of the entire total molecule. The antibody does not bind to the alpha-chain. Cross-reaction with LH, TSH and FSH can be neglected in immunohistochemistry, as this was < 0.8% in the radioimmunoassay.

E009 can be used to detect normal trophoblasts as well as HCG-secreting cells in trophoblastic neoplasms and chorionic carcinomas and in metastases derived from such tissues. HCG may also be expressed by aggressively growing carcinomas of the gastrointestinal and respiratory tracts and the breast. It is also present in chorionic carcinomas in the male testis and in germ cell tumors in the brain tissue/ cerebrospinal fluid of patients.

Pretreatment

Formaldehyde-fixed tissues do not need to be pretreated with proteolytic enzymes

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Placenta

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Vaitukaitis J.L., Braunstein G.D., Ross G.T.: A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of humen luteinizing hormone. Am.J.Obstet.Gynecol. 113, 751ff (1972)
  • Boronow R.C.: Gestational trophoblastic disease. In: Rutledge F., Boronow R.C., and Wharton J.T. eds. Gynecologic Oncology. NewYork, John Wiley and Sons (1976)
  • Waldmann T.A., Javadpour N., Glatstein E.: Testicular germ-cell neoplasms: Recent Advances in Diagnosis and Therapy. NIH Conference.Ann.Med. 90, 373 (1979)
  • Seidl Ch., Lippert Ch., Grouls V. und Jellinghaus W.: Leiomyosarkom des Samenstranges mit paraneolastischer ß-HCG Produktion. Pathologe 19, 146-150 (1998)
Specificity

hCG Beta (Chorionic Gonadotropin Beta)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

SC1
Antigen

Human hCG Beta (Chorionic Gonadotropin Beta)
Quantity

6 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet