HEA 125 (CD326; Ep-CAM)

Mouse anti-Human HEA 125 (CD326 EpCAM) (unconjugated), Clone HEA125, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E022
Category
Quantity
5 ml
Buy at BIOZOL

Background

The HEA 125 antibody recognizes an antigen that is a glycoprotein with a molecular weight of 34 kD and is expressed on the cell surface of most epithelial cell types.
If conversion from normal to neoplastic cells takes place, the expression of these proteins is maintained.

HEA125 is an excellent marker for the study of epithelial and mesothelial structures.

Synonyms

EP-CAM, Epithelial Glycoprotein 314, MK1, CD326, TACSTD1

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® HEA 125 (Bottle, 5 ml)

Antigen

Human epithelial specific antigen (HEA 125)

Specificity

All human epithelial cell types (exceptions: see Tab.1)
All carcinoma cell lines tested so far especially colon carcinoma cell lines (HT-29, WiDr, SW1116)

Immunogen

HAT-29 colon carcinoma cell line

Abnormal Tissue

All carcinoma types

Clone

HEA 125

Isotype

Mouse lgG1

This antibody reacts with all human epithelial cell types, in particular with all intestinal epithelia and all glandular and ciliated epithelia (except epidermis, sebaceous glands and cortical thymus epithelium). Furthermore, reaction occurs with all colon carcinoma cell lines and metastases derived from epithelia tested to date (see Tables 1 and 2). HEA 125 does not react with mesothelial cells. Keratinizing areas of a tumor mass usually remain unstained, as do sarcomas, lymphomas, melanomas, and neurogenic tumors

The E022 antibody is suitable for differentiating between carcinoma and non-carcinoma in both cryostat sections and formalin-fixed kerosene sections.

Pretreatment

Proteolytic pretreatment with 0.1% Pronase (LINARIS CatNo E110) 10 min at room temperature or HISTOPRIME-ENHANCER (LINARIS CatNr E7000)10 min at 96-100°C in a water bath or in a microwave oven

Incubation Period

1 hour at room temperature after pretreatment or overnight at 37°C. (without proteolytic pretreatment)

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Appendix or colon carcinoma

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase
Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Edwards P.A.W.: Heterogeneous expression of cell-surface antigens in normal epithelia and their tumors, revealed by monoclonal antibodies. Br. J. Cancer 51,149 (1985)
  • Momburg F., Moldenhauer G., Hämmerling G.J., Möller P., Otto H.F.: Charakterisierung eines epithelspezifischen Zellmembranantigens mit Hilfe des monoklonalen Antikörpers HEA 125. Verh. Dtsch. Ges. Path. 69,627 (1985)
  • Möller P., Momburg F., Moldenhauer G.: Epitheliale Membranmarker-Bestandsaufnahme, eigene monklonale Antikörper und aktuelle Möglichkeiten der Anwendung in der Histopathologie. Verh. Dtsch. Ges. Path. 70,116-126 (1986)
  • Moldenhauer G., Momburg F., Möller P., Schwartz R., Hämmerling G.J.: Epithelium-specific surface glycoprotein of Mr 34000 is a widely distributed human carcinoma maker. Br. J. Cancer 56,714-721(1987)
  • Momburg F., Moldenhauer G., Hämmerling G.J., Möller P.: Immunohistochemical study of the expression of a Mr 34000 human epithelium specific surface glycoprotein in normal and malignant tissues. Cancer Research 47,2883-2891 (1987)
  • Hastka J., Pfiester P.: Zellmarker zur Differentialdiagnose maligner Mesotheliome. Pathologe 9,245-247 (1988)
  • Bohrer M.H., Hastka J., Verbeke C.S.: Differentialdiagnose von hepatischen Tumormanifestationen in der Tumordiagnose und Therapie 13,187-189 (1992)
HEA 125 (CD326; Ep-CAM)
Immunohistochemical staining of CD326; Ep-CAM with antibody clone HEA 125
Specificity

HEA 125 (CD326 EpCAM)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

HEA125
Antigen

Human HEA 125 (CD326 EpCAM)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet