Mouse anti-Human Macrophages/Monocytes (unconjugated), Clone MAC387, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E026
Category
Quantity
5 ml
Buy at BIOZOL

Background Macrophages/Monocytes

The monoclonal antibody recognizes the cytoplasmic antigen L1 (calprotectin) of the monocyte/macrophage lineage. It is expressed by neutrophil granulocytes as well as tissue histiocytes, alveolar macrophages, Kupffer cells, splenic macrophages, infiltrating macrophages, sinoidal macrophages as well as epithelial giant cells. It is also present in the cytoplasm of epthelial cells.

Synonyms

Macrophages/Monocytes Marker, cytoplasmic antigen L1, Calprotectin

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Macrophages/Monocytes (Bottle, 5 ml )

Specificity

Cytoplasmic antigen in cells of the monocyte/macrophage lineage (including histiocytes, alveolar macrophages, Kupffer cells, splenic macrophages, tumor-infiltrating macrophages, sinoid macrophages, and epithelial giant cells; also granulocytes under certain conditions.

Abnormal Tissue

Monocytes and macrophages in malignant tumors.

Clone

MAC387

Isotype

Mouse lgG1

Fusion Partners

BALB/c mice were immunized with purified blood monocytes. Spleen cells from these animals were fused with mouse myeloma cell line NS-1. The monoclonal antibody is available in readyto-use form.

The monoclonal antibody E026 (MAC 387) stains the cytoplasm of cells of the monocytemacrophage lineage. For example, infiltrating and reactive histiocytes or tumor-infiltrating macrophages can be detected.

Pretreatment

proteolytic pretreatment with 0.1% Pronase (LINARIS CatNo E110) 10 min at room temperature

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Lymph nodes, spleen or tonsils

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • David J. Flavell, David B. Jones, and Dennis H. Wright (1987I Indentification of Tissue Histocytes on Paraffin Sections by a New Monoclonal Antibody. J. Histochem. and Cytochem.35, (11), 1217-1226.
  • Brandtzaeg P., Jones D.B., Flavell D.J., Fagerhold M.K. (1988) MAC387 Antibody and Detection of Formalin-resistant Myelomonocytic L1 Antigen.J.Clin. Pathol. 41; 963-970
  • Brandtzaeg P., Dale I., Gabrielsen T-O. (1992) The Leucocyte Protein L1 (Calprotectin): Usefulness as an Immunohistochemical Marker Antigen and Putative Biological Function and Putative Biological Function. Histopathol. 21; 191-196
Specificity

Macrophages/Monocytes
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

MAC387
Antigen

Human Macrophages/Monocytes
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet