Mouse anti-Human Macrophages/Monozytes (unconjugated), Clone MAC387, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K026
Category
Quantity
0, 1 ml
Buy at BIOZOL

Background Macrophages/Monocytes

The myeloid/histiocyte antigen recognized by the monoclonal antibody Mac 387 (1) is an abundant cytoplasmatic granulocyte protein with a molecular mass of 36 kDa (2). It is also present in some other cell types e.g. monocytes, certain reactive tissue macrophages, squamous mucosal epithelium and reactive epidermis (4-6). The protein (2) has several designations among which S100A8/S100A9 indicates that it belongs to the S100 protein family according to the recently established nomenclause for this family (3). The protein contains at least two different subunits which have molecular masses of 8 and 14 kDa, respectively. The whole molecule is thus a heterooligomer consisting of two or three subunits (2,3,8).

Synonyms

Macrophages/Monocytes Marker, cytoplasmic antigen L1, Calprotectin

Volume/Quantity

0.5 ml

Product Form

Tissue culture supernatant – liquid

Preparation

Tissue culture supernatant (RPMI 1640 medium containing fetal calf serum)

Clone Number

Mac387

Buffer

0.05 mol/L Tris/HCL pH7.2

Approx. Protein Concentrations

IgG concentration 0,43 mg/ml

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)

Immunogen

Purified peripheral blood monocyte components prepared by affinity chromatography on polyclonal antimonocyte-Sepharose 6B.

Isotype

IgG1 (Mouse)

Specificity

The Mac 387 antibody labels the cytoplasma of many myelomonocytic cells and reacts with a predominating subset of reactive macrophages. A positive reaction is also seen in several cases of histiocytosis both the malignant and the haemophagocytic types (4,7). In blood smears from peripheral blood a positive staining of monocytes and granulocytes, but not lymphocytes, is seen.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/50-1/100
Immunohistology-paraffin Yes 1/50-1/100
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested

Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections with 10 mmol/L citrate buffer pH6.0.

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C. DO NOT FREEZE!
Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Flavell DJ, Jones DB, Wright DH. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem 1987;35:1217-26.

Fagerhol MK. Nomenclature for proteins: is calprotectin a proper name for the elusive myelomonocytic protein? J Clin Pathol: Mol Pathol 1996:49:74-9.

Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. TIBS 1996;21:134-40.

Brandtzaeg P, Jones DB, Flavell DJ, Fagerhold MK. Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 1988;41:963-70.

Jones DB, Flavell DJ, Wright DH. M4.12. Tissue reactivity of a monoclonal antibody directed against cells of the human monocyte-macrophage lineage in formalin-fixed, paraffin-embedded tissue. In: McMichael AJ et al., eds. Leucocyte Typing III. White Cell Differentiation Antigens. Oxford – New York Tokyo: Oxford University Press, 1987:692-4.

Davey FR, Gatter KC, Ralfkiaer E, Pulford KAF, Krissansen GW, Mason DY. Immunophenotyping of non-Hodgkin’s lymphomas using a panel of antibodies on paraffin-embedded tissues. Am J Pathol 1987;129:54-63.

Brandtzaeg P, Dale I, Gabrielsen T-Ø. The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 1992;21:191-6.

Siegenthaler G, Roulin K, Chatellard-Gruaz D, Hotz R, Saurat JH, Hellman U et al. A heterocomplex formed by the calcium-binding proteins MRP8 (S100A8)

Specificity

Macrophages/Monozytes
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

MAC387
Antigen

Human Macrophages/Monozytes
Quantity

0, 1 ml
Format

Purified
Storage Temperature

-20 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet