Mouse anti-Human Melanoma (unconjugated), Clone HMB45, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E050
Category
Quantity
5 ml
Buy at BIOZOL

Background

Using monoclonal antibodies, it is possible to identify numerous melanoma-associated antigens. These are expressed on malignant melanomas and their metastases. The antigens themselves have been poorly defined to date.

The melanoma marker HMB45 is expressed by malignant melanomas, reactivated melanocytes, junctional and blue nevus cells, regardless of the degree of pigmentation. Normal melanocytes and other neoplasms are not detected by this maker.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Melanoma (Bottle, 5 ml)

Antigen

Melanoma-associated antigen

Specificity

Melanoma, junctional nevi, embryonic melanocytes.

Clone

HMB45

Isotype

Mouse lgG1

Fusion Partners

The monoclonal antibody was obtained from culture supernatant of mouse hybridoma cells.

Most melanomas are stained by this antibody. In contrast, epithelial, lymphoid or glial tumor cells are negative. The monoclonal antibody also reacts with antigen on fetal and neonatal melanocytes.

Pretreatment

Proteolytic pretreatment with 0.1% pronase (LINARIS Cat. No. E110) for 10 minutes at room temperature.

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Melanoma

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Gown, A. M. et al. Monoclonal antibody spezific for melanocytic turmors distinguish subpopulations of melanocytes. Am. J. Pathol. 90, 385 (1988)
  • Ordonez, N. G. et al. Comparison of HMB45 monoclonal antibody and S-100 protein in the immunohistochemical dignosis of melanoma. Am. J. Clin. Pathol. 90, 385 (1988)
  • Wick M. R., et al. Immunohistochemical diagnosis of sinonasal melanoma , carcionma, and neuroblastoma with monoclonal antibodies HMB45 and anti-synaptophysin. Arch. Path. Lab. Med. 112, 616 (1988)
Specificity

Melanoma
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

HMB45
Antigen

Human Melanoma
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet