Mouse anti-Human Mesothelial Cell (unconjugated), Clone HBME-1, IgM, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E071
Category
Quantity
5 ml
Buy at BIOZOL

Background

In immunohistochemistry, the monoclonal antibody E071 stains mesothelial cells. It recognizes an antigen localized on the plasma membrane of mesothelial cells.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Mesothelial cells (Bottle, 5 ml)

Antigen

A suspension of human mesothelioma cells from patients with malignant epithelial mesothelioma

Abnormal Tissue

Mesothelioma

Clone

HBME-1

Isotype

Mouse lgGM

This monoclonal antibody reacts with 17/19 epithelial mesotheliomas in a characteristic pattern of a thick appearing membrane. This staining pattern correlates well with the presence of many lung microvilli on the membrane surface of these cells.
Although 19 of 50 adenocarcinomas of diverse origin also stain positive, their staining pattern is
often cytoplasmic. In individual cases, a thinly stained membrane appears.

Pretreatment

No proteolytic enzymes required

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).

Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Miettinen M and Kovatich AJ. HMBE-1 a monoclonal antibody useful in the differential diagnosis of mesothelioma, adenocarcinoma, and softtissue and bone tumors. Appl Immunohistochem 1995; 3(2):115
Specificity

Mesothelial Cell
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgM
Application

IHC-F, IHC-P
Clone

HBME-1
Antigen

Human Mesothelial Cell
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet