Mouse anti-Human Neuron Specific Enolase (NSE) (unconjugated), Clone MiG-N3, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E023
Category
Quantity
5 ml
Buy at BIOZOL

Background Neuron Specific Enolase (NSE)

Enolase is an enzyme of glycolysis and catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate. The cytoplasmic enzyme exists in 3 subunits that occur as homo- (alpha-alpha, beta-beta, gamma-gamma) or heterodimers (alpha-beta, alpha-gamma) in the 5 known isozymes. In liver and gila, alpha-alpha enolase is found, and in skeletal and cardiac muscle, alpha-beta and beta-beta enolase. In contrast, in brain a mixture are the subunits alpha and gamma as – alpha-alpha, alpha-gamma and gamma-gamma dimers. The monoclonal antibody E023 recognizes human gamma-gamma enolase with a molecular weight of about 100kDa.

Synonyms

Neuron-specific enolase (NSE)

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® NSE (Bottle, 5 ml)

Antigen

Human gamma-Enolase

Specificity

Gamma-Enolase

Abnormal Tissue

Mainly neuronal and neuroendocrine tumors*.

Clone

MIG-N3

Isotype

Mouse lgG1

Fusion Partners

BALB/C mice were immunized with purified human gamma-gamma enolase. Spleen cells from these animals were fused with a mouse myeloma cell line. The resulting hybridoma cells were used for ascites recovery. The specificity of the purified antibody was tested in enzyme immunoassays as well as immunohistochemically on various normal and tumor tissues.

Normal Tissue

Axons, dendrites and cell bodies (perikaries) of brain neurons as well as peripheral nerve fibers and ganglia, adrenal medulla, pancreatic islet cells and other cells of neuroectodermal origin are positive for NSE.

Abnormal Tissue

NSE antibodies serve predominantly as markers of tumors of the nervous system and diffuse neuroendocrine system (APUD system). For example, neuroblastomas, astrocytomas, glioblastomas, meningiomas, and pituitary adenomas are positive. In addition, melanoma, medullary thyroid carcinoma, and small cell lung carcinoma (SCLC) usually stain with gamma enolase.

Pretreatment

Pretreatment with proteolytic enzymes not required. In the case of formaldehyde-fixed material, usually significant improvement of results with HISTOSAFE-ENHANCER (LINARIS CatNo E7000).

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Nervous tissue or pancreas

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and SubstrateKit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

Cooper, E.H. Splinter, T.A.W., Brown, D.A., Muers, M.F., Peake, M.D., and Pearson,S.L. (1985) Evaluation of radioimmunoassay for neuron specific enolase in small cell lung cancer. Br.J. Cancer 52; 333-338

Haimoto, H., Takahashi, Y., Koshikawa, T., Nagura, H., and Kato, K. (1985) Immunhistochemical localization of gamma-enolase in normal human tissues other than nervous and neuroendocrine tissues. Lab. Invest. 52,3; 257-263

Soler Federsppiel, B.S., Cras, O., Gheuens, J., Andries, D., and A. Lowenthal (1987) Human gamma-gamma-Enolase: Two-site immunoradiometric assay with a single monoclonal antibody. J. Neurochem. 48; 22-28

Cras, P., Martin, J.J., and Gheuens, J. (1988) Gamma-Enolase and glial fibrillary acidic protein in nervous system tumors: An immunohistochemical study using specific monoclonal antibodies. Acta Neuropathol. 75; 377-384

Seshi, B., True, L., Carter, D., and Rosai, J. (1988) Immunohistochemical characterization of a set of monoclonal
antibodies to human neuron-specific enolase. Am J. Pathol. 131,2; 258-269

Specificity

Neuron Specific Enolase (NSE)
Species Reactivity

Guinea Pig, Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

MiG-N3
Antigen

Human Neuron Specific Enolase (NSE)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet