Mouse anti-Human Neuron Specific Enolase (NSE) (unconjugated), Clone MiG-N3, IgG1

Concentrated Antibody for Immunohistochemistry

SKU:
K023
Category
Quantity
0, 5 ml
Buy at BIOZOL

Background Neuron Specific Enolase (NSE)

Enolase is an enzyme of glycolysis and catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate. The cytoplasmic enzyme exists in 3 subunits that occur as homo- (alpha-alpha, beta-beta, gamma-gamma) or heterodimers (alpha-beta, alpha-gamma) in the 5 known isozymes. In liver and gila, alpha-alpha enolase is found, and in skeletal and cardiac muscle, alpha-beta and beta-beta enolase. In contrast, in brain a mixture are the subunits alpha and gamma as – alpha-alpha, alpha-gamma and gamma-gamma dimers. The monoclonal antibody E023 recognizes human gamma-gamma enolase with a molecular weight of about 100kDa.

Synonyms

Neuron-specific enolase (NSE)

Volume/Quantity

500 µl

Product Form

Ascites – liquid

Clone Number

MIG-N3

Buffer

Phosphate buffered saline pH7.6

Approx. Protein Concentrations

IgG concentration 12 mg/ml

Preservatives Stabilisers

0,09% Sodium Azide (NaN3)
1% Bovine Serum Albumin (BSA)

Immunogen

Purified human gamma enolase

Isotype

IgG1 (Mouse)

Specificity

In normal tissues, most neurons and their axonal and dendritic processes stain strongly positive for NSE, with the exception of Purkinje’s cells. Schwann’s cells, cells of the adrenal medulla and paraganglia also contain NSE. Endocrine cells of the skin (Merkel’s cells), respiratory and GI tract epithelium, pituitary parathyroid, pancreas islets, and C cells of the thyroid all stain positive for NSE.
NSE has been demonstrated in ganglioneuromas, neuroblastomas, Schwannomas and malignant melanomas. It is also present in pheochromocytomas and paragangliomas. Carcinoids, medullary thyroid carcinomas, pituitary adenomas, and endocrine tumors of the pancreas and GI tract all show positive immunoreactivity for NSE. NSE is found in neuroendocrine carcinoma of the skin (Merkel’s cell tumor) and small cell carcinoma of the lung. A number of non-neuronal and nonendocrine tumors may also express NSE, including meningiomas, astrocytomas, renal cell carcinomas, carcinoma and fibroadenoma of the breast, carcinoma of the ovary, and malignant lymphomas. NSE positivity should not be relied upon entirely as evidence of the neuronal or neuroendocrine differentiation of a given neoplasm.

Fusion Partners

Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse NSI myeloma cell line.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/100 – 1/200
Immunohistology-paraffin Yes 1/100 – 1/200
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

This product does not require protein digestion pre-treatment of paraffin sections

Positive Control Tissue

Intestine or Pancreas

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Westernblotting

Chemiluminescent Substrate

DuoLuX Chemiluminescent Substrate for Alkaline Phosphatase (AP) (LINARIS CatNo ESC6605) and Peroxidase (POD) (LINARIS CatNo ESC6604).

Western Blotting Immunodetection Kit

ABC-AmP Chromogenic Detection Kit anti-Mouse IgG (LINARIS CatNo EDA6402) or anti-Rabbit IgG (LINARIS CatNo EDA6401)

Weight Standard

Biotinylated protein molecular weight marker (LINARIS CatNo ESP1400). Molecular weight range from
19kD to 222kD.

Storage Conditions

Store at 2-8°C. DO NOT FREEZE!
Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

DeStephano, D.B., Lloyd, R.V., Pike, A.N., Wilson, B.S. (1984). Pituitary Adenomas: An immunohistochemical study of hormone production and chromogranin. Am. J. Pathol. 116: 464.

Lloyd, R.V. and Wilson, B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody. Science 222: 628

Lloyd R.V., Mervak T., Schmidt K., Warner T., Wilson B.S. (1984). Immunohistochemical detection of chromogranin and neuron-specific enolase in pancreatic endocrine neoplasms. Am. J. Surg. Pathol. 8: 607.

Walts, A.E., Said, J.W., Shintaku, P. and Lloyd, R.V.: (1985). Chromogranin as a marker of neuroendocrine cells in cytologic material – an immunocytochemical study. Am. J. Clin. Pathol. 84: 273-277.

Wilson, B.S., and Lloyd, R.V. (1984). Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115: 458.

Specificity

Neuron Specific Enolase (NSE)
Species Reactivity

Guinea Pig, Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

MiG-N3
Antigen

Human Neuron Specific Enolase (NSE)
Quantity

0, 5 ml
Format

Concentrate
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet