Mouse anti-Human p53 (unconjugated), Clone DO-7, IgG2b, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E052
Category
Quantity
5 ml
Buy at BIOZOL

Background

The p53 tumor suppressor gene is one of the most frequently altered genes in malignant tumors of human tissues. P53 is a transcription factor that regulates cell growth and prevents cells from entering the S phase of mitosis. The p53 oncoprotein is expressed at very low levels in normal cells. However, common point mutations on chromosome 17 can lead to accumulation of the mutant phosphoprotein. The expression product of the mutant gene no longer has binding ability for DNA and thus no suppressor activity.
The p53 protein is expressed in a large number of different carcinomas. For colorectal carcinomas with a high expression rate, a study on formaldehyde-fixed material after treatment with “Target Unmasking Fluid” showed a sensitivity of 67% and a specificity of 90% for immunohistochemical detection with clone DO-7 (corresponding to E052) (Baas et al., 1994).

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® p53 (Bottle, 5 ml)

Antigen

p53 phosphoprotein

Specificity

N-terminus of the p53 protein

Clone

DO-7

Isotype

Mouse lgG2b

Fusion Partners

The monoclonal antibody was obtained from culture supernatant of mouse hybridoma cells.

The monoclonal antibody E052 recognizes the N-terminus of the human p53 protein. It reacts with both the normal and mutant forms of the protein. The antibody can be used on cryostat material and formaldehyde-fixed kerosene material.
For kerosene sections, pretreatment with pronase is necessary, but this can advantageously be replaced by unmasking with HistoSafe enhancer (Linaris CatNo E7000).

Pretreatment

Enzymatic pretreatment with 0.1% Pronase (Linaris CatNo E110) for 10 minutes at room temperature.
Better results can be achieved by pretreatment HISTOSAFE-ENHANCER (Linaris CatNr E7000) 10 minutes at 96°C (water bath), 100°C (pressure cooker) or 2 x 5 minutes in temperature controlled microwave:

Incubation Period

1 hour by room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Breast Cancer

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Cattoretti G., Rilke F., Andreola S., D’Amato L., and Delia D. p53 expression in breast cancer. Int. J. Cancer 41: 178-183 (1988)
  • Nigro M. J., Baker S. J., Preisinger A. C., Jessup J. M., Hostetter R., et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-708 (1989)
  • Van den Berg F. M., Tigges A. J., Schipper M. E. I., Den Hartog-Jager F. C. A., Kroes W. G. M., Walboomers J. M. M. Expression of the nuclear oncogene p53 in colon tumours. : Clin. Pathol. 157: 193-199 (1989)
  • Iggo R., Gatter K., Bartek J., Lane D., Harris A. L., Increased expression of mutant forms of p53 oncogene in lung cancer. Lancet 335: 675-679 (1990)
  • Bartek J., Bartkova J., Voitesek B., Staskova Z., Lukas J., Rejthar A., et al. Aberrant expression of the p53 oncoprotein is a common
    feature of a wide spectrum of human malignancies. Oncogene 6: 1699-1703 (1991)
  • Baas, I. O., Mulder J-W. R., Johan G., Offerhaus A., Vogelstein B., and Hamilton S. R. Am evaluation of six antibodies for immunocytochemistry of mutant p53 gene product in archival colorectal neoplasms. J. Patho. 172: 5-12 (1994)
Specificity

p53
Species Reactivity

Bovine, Human
Host / Source

Mouse
Isotype

IgG2b
Application

IHC-F, IHC-P
Clone

DO-7
Antigen

Human p53
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet