Mouse anti-Human Progesterone Receptor (PGR-R) (unconjugated), Clone 1A6, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K054
Category
Quantity
0, 5 ml
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Background

Steroid hormone receptors are of great importance for the diagnosis and therapy of breast cancer. The expression of estrogen or progesterone receptors in tumor tissue is a prerequisite for a patient’s response to anti-hormone therapy.

Volume/Quantity

0.5 ml

Product Form

Culture Supernatant – liquid

Preservatives Stabilisers

15 mM Sodium Azide (NaN3)

Immunogen

Synthetic peptide corresponding to a site of predicted high antigenicity on the human progesterone receptor.

Clone Number

1A6

Isotype

IgG1 (Mouse)

Specificity

K054 is recommended for determining the progesterone receptor alpha status of breast cancer tissue.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Not tested
Immunohistology-paraffin Yes 1/40
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Yes 1/25-1/50
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Breast Cancer

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Specificity

Progesterone Receptor (PGR-R)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

1A6
Antigen

Human Progesterone Receptor (PGR-R)
Quantity

0, 5 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet