Mouse anti-Human PSA (Prostatic Specific Antigen) (unconjugated), Clone ER-PR8, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K040
Category
Quantity
0, 5 ml
Buy at BIOZOL

Background

The anti-Prostate Specific Antigen (PSA) monoclonal antibody recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). It is highly specific for PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues.

Volume/Quantity

0.5 ml

Product Form

Purified IgG – liquid

Buffer

0.01 M Phosphate buffered saline

Preservatives Stabilisers

0.09% Sodium Azide (NaN3)
1% Bovin Serum Albumin (BSA) as carrier protein.

Immunogen

Purified human PSA

Clone Number

ER-PR8

Isotype

IgG1, kappa (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/25-1/50
Immunohistology-paraffin Yes 1/25-1/50
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Pre-treatment

Not required.

Positive Control Tissue

Prostate

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Nap M, van der Kwast TM. Immunohistochemical characterization of 53 monoclonal antibodies to prostate-specific antigen. Tumor Biol 1999 (suppl. 1):20:38-42.

Papadopoulos I, Sivridis E, Giatromanolaki A, Koukourakis MI. Tumor angiogenesis is associated with MUC1 overexpression and loss of prostate-specific antigen expression in prostate cancer. Clin Cancer Res 2001:7;1533-8.

Polascik TJ, Oesterling JE, Partin AW. Prostate specific antigen: a decade of discovery – what we have learned and where we are going [Review Article]. J Urol 1999:162;293-306.

Stenman U-H, Leinonen J, Zhang W-M, Finne P. Prostate-specific antigen. Cancer Biol:1999;9:83-93.

Wang TJ, Linton HJ, Sokoloff RL, Grauer LS, Rittenhouse HG, Wolfert RL. Western blotting analysis of antibodies to prostate-specific antigen: specificities for prostate-specific antigen and prostatespecific antigen fragments. Tumor Biol 1999;20 (suppl 1):79-85.

Stenman U-H, Paus E, Allard WJ, Andersson I, Andrés C, Barnett TR, et al. Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen. Tumor Biol 1999;20 (suppl 1): 1-12.

Riesenberg R, Oberneder R, Kriegmair M, Epp M, Bitzer U, Hofstetter A, et al. Immunocytochemical double staining of cytokeratin and prostate specific antigen in individual prostatic tumour cells. Histochemistry 1993;99:61-6.

Alanen KA, Kuopio T, Koskinen PJ, Nevalainen TJ. Immunohistochemical labelling for prostate specific antigen in non-prostatic tissues. Path Res Pract 1996;192:233-7.

Specificity

PSA (Prostatic Specific Antigen)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

ER-PR8
Antigen

Human PSA (Prostatic Specific Antigen)
Quantity

0, 5 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet