Mouse anti-Human Vimentin (unconjugated), Clone V9

Ready-to-use Antibody for Immunohistochemistry

SKU:
E034
Category
Quantity
5 ml
Buy at BIOZOL

Background

Vimentin is a protein with a molecular mass of 57kD. It forms, together with microfilaments and microtubules, the cytoskeleton of non-epithelial cells, e.g. non-muscular mesenchymal cells and cells of mesenchymal origin including vascular smooth muscle, astrocytes and numerous cultured cell lines. It frequently occurs together with desmin.
Expression of this protein is retained during the conversion of normal to neoplastic cells.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® Vimentin (V9) (Bottle, 5 ml)

Antigen

von Willebrand Factor isolated from human plasma

Specificity

von Willebrand Factor (factor VIII-associated antigen)

Abnormal Tissue

Vimentin experimenting tumors including sarcomas, lymphomas, melanomas

Clone

V9

Isotype

Mouse lgG1

Fusion Partners

BALB/c mice were immunized with vimentin (from pig lentils). Spleen cells from these animals were fused with a tumor cell line. The resulting hybridoma cells were used for ascites production.

The monoclonal antibody E034 reacts with human vimentin as well as with vimentin in pig, chicken and rat. It recognizes tumors of mesenchymal origin, e.g. sarcomas and malignant lymphomas.
Importantly for its application, this antibody does not require pretreatment with pronase. For formaldehyde-fixed material, the intensity of the staining may be indicative of the fixation time.

To be independent of this, preparation with HISTOSAFE-Enhancer E7000 may be usefu

Pretreatment

Pre-treatment with proteolytic enzymes not required!
HISTOAFE-ENHANCER (LINARIS CatNo. E7000 can improve the results).

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on
formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Prostate or appendix

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109 ).

  • Lazarides, E., Nature 283, 249 – 256 (1980)
  • Franke, W.W., E. Schmid, S. Winter, M. Osborn, K. Weber, Exp. Cell Res.123, 25-46 (1979)
  • Lazarides, E., Ann.Rev.Biochem.51, 219-250 (1983).
  • Osborn, M., K. Weber, Laboratory Investigation 48, 372-394 (1983)
  • Toelle, H.-G., K. Weber, M. Osborn, M Eur.Cell Biol.38, 2334-244 (1985)
  • Heid, H.W. , Moll I., and Franke, W.W. Differentiation 37, 137-157 (1988)
Specificity

Vimentin
Species Reactivity

Chicken, Guinea Pig, Human, Rabbit, Rat, Swine
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

V9
Antigen

Human Vimentin
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet