Mouse anti-Human von Willebrand Factor (Faktor VIII) (unconjugated), Clone F8/86, IgG1, Ready-to-use

Ready-to-use Antibody for Immunohistochemistry

SKU:
E033
Category
Quantity
5 ml
Buy at BIOZOL

Background

The factor VIII complex is a protein found in normal human plasma and belongs to the coagulation factors. It consists of 3 subunits: factor VIII RCF (ristocitin cofactor), factor VIIIC (coagulant portion) and factor VIII-associated antigen (Von Willebrand factor). This represents the large-molecule portion of the factor VIII complex. Its formation occurs in blood platelets and in the endothelium of all vascular segments, probably also in the RES of the liver. It is also found in megakaryocytes.

Reagents sufficient for about 50-100 tissue sections

  • 1 dropper bottle HISTOPRIME® von-Willebrand-Factor (Bottle, 5 ml)

Antigen

von Willebrand Factor isolated from human plasma

Specificity

von Willebrand Factor (factor VIII-associated antigen)

Normal Tissue

Human endothelial cells, megakaryocytes

Clone

F8/86

Isotype

Mouse lgG1

Fusion Partners

(C57BL/6xBalb/c) F1 mice were immunized with factor VIII-associated antigen. Splenocytes from these animals were fused with myeloma cell line NS1. The antibody is available as culture supernatant of the resulting hybridoma cells.

The monoclonal antibody against Von Willebrand factor E033 is used to stain (cytoplasmic, granular) endothelial cells and megakaryocytes in human tissue. Tumors originating from endothelial cells or megakaryocytes can be deployed by detecting this antigen. In addition, vascularization within different tissues can be assessed and tumor invasion into blood vessels can also be detected.

Pretreatment

Proteolytic pretreatment with 0.1% Pronase (Cat.No.E110), 10 minutes at room temperature.

Incubation Period

1 hour at room temperature

Usage

Ready-to-use in PBS, BSA, NaN3 (0,09%) pH 7,4(*) suitable on cryostat sections and on
formalin-fixed, paraffin-embedded tissue sections.

Control Tissue

Goiter, colon carcinoma, appendix

Recommended Secondary Reagents

Alkaline Phosphatase Vectastain® ABC Mouse IgG (Vector CatNo AK-5002) and Substrate-Kit e.g. Vector® Red (Vector CatNo SK-5100).
Peroxidase Vectastain® ABC-Elite Mouse IgG (Vector CatNo PK-6102) and Peroxidase Substrate-Kit e.g. DAB (LINARIS CatNo E108) or HistoGreen (LINARIS CatNo E109).

  • Naiem M., Gerdes J., Abdulaziz Z. et al. (1982) The value of immunohistological screening in the production of monoclonal antibodies.
    J. Immunol. Meth. 50; 145 – 60.
  • Avuer P., Armand D., Sultau Y., Maisonnenve P-Jeanneau C. (1983) AISP Symposium, Larger Ed.: Monoclonal antibodies against
    the human factor VIII Willebrand molecule, characterization and potential for screening of Willebrand patiens.
  • Hruban R.H., Kuhajda F.P., and Mann R.B. (1987) Acute myelofibrosis. Immunohistochemical study of four cases and comparison
    with acute megakaryocytic leukemia. Am. J. Clin. Pathol. 88; 578 – 588.
  • Mejan O., Fert V., Delezay M., Delage M., Cluballah R., and Bourgois A. (1988) Thrombosis and Haemostasis: Immunopurification
    of human factor VIII/vWF complex from plasma. 59, 364 – 371
Specificity

von Willebrand Factor (Faktor VIII)
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

F8/86
Antigen

Human von Willebrand Factor (Faktor VIII)
Quantity

5 ml
Format

RTU
Storage Temperature

2-8 °C
Shipping Temperature

20 °C

Documents

pdf Datasheet